γδ T cell versus NK cell function in intestinal immunity

γδ T 细胞与 NK 细胞在肠道免疫中的功能

• 批准号: 453662420
• 负责人: Professor Dr. Thomas Göbel
• 金额: --
• 依托单位: Lehrstuhl für Tierphysiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/453662420?language=en
• 关键词: cell; versus; NK; function; intestinal

Chickens belong to the γδ high species with a frequency of up to 50 % of circulating T cells. Their function is largely unknown and there are only very few markers that can be used for phenotypic analyses of γδ T cells. Moreover, there is only sparse information regarding the TCR usage in various tissues and changes of γδ T cell repertoire following infections. Work package 4 will address several research questions regarding the phenotype and function of γδ T cells. In particular, we will analyze similarities and differences between intestinal NK cells and γδ T cells that are both highly abundant intestinal cell populations. In addition, will characterize novel γδ T cell specific mAb and use them in diverse infection experiments to explore different γδ T cell subpopulations and/or activation states. These novel tools will be freely available for the other research projects to analyze phenotypic changes of γδ T cells during various disease models. We will also use our newly developed in vitro culture system for γδ T cell to functionally characterize these cells and their interaction with pathogens in a defined system. In addition, we will setup tools to monitor the γδ T cell repertoire in order to monitor changes of the TCR repertoire during in vitro culture but also in infection models. The individual research projects within FOR5130 benefit from the collaborations among each other and provide a better insight into the immune system of chickens in relation to important pathogens.

鸡属于γδ高发种，其频率高达循环T细胞的50%。它们的功能在很大程度上是未知的，可以用于γδT细胞表型分析的标记物也很少。此外，关于TcR在各种组织中的使用以及感染后γδT细胞库的变化的信息也很少。工作包4将解决有关γδT细胞的表型和功能的几个研究问题。特别是，我们将分析肠道NK细胞和γδT细胞的异同，这两种细胞都是高度丰富的肠道细胞群。此外，还将鉴定新的γδT细胞特异性单抗，并将其用于不同的感染实验，以探索不同的γδT细胞亚群和/或激活状态。这些新的工具将免费提供给其他研究项目，以分析各种疾病模型中γδT细胞的表型变化。我们还将使用我们新开发的γδT细胞体外培养系统来确定这些细胞的功能特征以及它们与病原体在特定系统中的相互作用。此外，我们将设置工具来监控γδT细胞库，以监控TCRT细胞库在体外培养过程中以及在感染模型中的变化。FOR5130中的个别研究项目受益于彼此之间的合作，并提供了对鸡与重要病原体相关的免疫系统的更好了解。