γδ T cell versus NK cell function in intestinal immunity

鸡属于γδ高种类，循环T细胞的频率高达50%。它们的功能在很大程度上是未知的，只有很少的标记物可以用于γδ T细胞的表型分析。此外，关于TCR在各种组织中的使用和感染后γδ T细胞库的变化，只有稀疏的信息。工作包4将解决有关γδ T细胞的表型和功能的几个研究问题。特别是，我们将分析肠道NK细胞和γδ T细胞之间的异同，两者都是高度丰富的肠道细胞群。此外，我们将对新型γδ T细胞特异性mAb进行表征，并将其用于不同感染实验，以探索不同的γδ T细胞亚群和/或激活状态。这些新工具将免费提供给其他研究项目，以分析各种疾病模型中γδ T细胞的表型变化。我们还将使用我们新开发的γδ T细胞体外培养系统来功能表征这些细胞及其在定义系统中与病原体的相互作用。此外，我们将建立工具来监测γδ T细胞库，以监测TCR库在体外培养和感染模型中的变化。FOR5130内的各个研究项目受益于彼此之间的合作，并提供了更好地了解鸡的免疫系统与重要病原体的关系。

Chickens belong to the γδ high species with a frequency of up to 50 % of circulating T cells. Their function is largely unknown and there are only very few markers that can be used for phenotypic analyses of γδ T cells. Moreover, there is only sparse information regarding the TCR usage in various tissues and changes of γδ T cell repertoire following infections. Work package 4 will address several research questions regarding the phenotype and function of γδ T cells. In particular, we will analyze similarities and differences between intestinal NK cells and γδ T cells that are both highly abundant intestinal cell populations. In addition, will characterize novel γδ T cell specific mAb and use them in diverse infection experiments to explore different γδ T cell subpopulations and/or activation states. These novel tools will be freely available for the other research projects to analyze phenotypic changes of γδ T cells during various disease models. We will also use our newly developed in vitro culture system for γδ T cell to functionally characterize these cells and their interaction with pathogens in a defined system. In addition, we will setup tools to monitor the γδ T cell repertoire in order to monitor changes of the TCR repertoire during in vitro culture but also in infection models. The individual research projects within FOR5130 benefit from the collaborations among each other and provide a better insight into the immune system of chickens in relation to important pathogens.