Study on the role of avian uncoupling protein in heat stress-induced overproduction of mitochondrial reactive oxygen species and the regulation mechanism of the expression in birds

鸟类解偶联蛋白在热应激诱导鸟类线粒体活性氧过剩中的作用及其表达调控机制研究

• 批准号: 23880003
• 负责人: KIKUSATO Motoi
• 金额: $ 2万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23880003/
• 关键词: 暑熱; 酸化ストレス; 脱共役タンパク質; 活性酸素; ミトコンドリア; プロトンリーク; 膜電位; 暑熱ストレス; UCP; オリーブオイル; オレウロペイン; 活性酸素種; 肉用鶏; 産卵鶏

On exposure of meat-type chickens to heat stress, avian uncoupling protein (avUCP) expression in the skeletal muscle was decreased, which was involved in the overproduction of mitochondrial reactive oxygen species production (ROS) probably via an increase in the mitochondrial membrane potential. This study demonstrates that the heat stress-induced overproduction of mitochondrial ROS did not occur in the muscle mitochondria of laying-type chickens, whose avUCP expression is relatively higher than that of meat-type ones, and in mitochondria of meat-type chickens whose avUCP expression was up-regulated with dietary intake of olive oil and oleuropein. From these results, it is conceivable that avUCP expression may play an important role in suppressing the overproduction of mitochondrial ROS and oxidative damage caused by heat exposure.

肉类鸡暴露于热应激时，骨骼肌中的鸟解偶联蛋白（AVUCP）的表达减少了，这与线粒体反应性氧（ROS）的生产过多有关，可能是通过线粒体膜电位的增加而导致的。这项研究表明，热应激诱导的线粒体ROS的过量产生在植物型鸡的肌肉线粒体中没有发生，它们的avucp表达相对较高，其肉质鸡的肉质鸡的表达相对较高。从这些结果中可以想象，AVUCP表达可能在抑制线粒体ROS的过量生产和热暴露引起的氧化损伤中起重要作用。

项目成果

Heat stress-induced overproduction of mitochondrial ROS is down-regulated in laying-type chickens

热应激诱导的产蛋鸡线粒体活性氧过量产生下调

• 发表时间: 2013
• 作者: Kikusato;M. and Toyomizu;M.
• 通讯作者: M.

産卵鶏は急性暑熱曝露時においても酸化ストレスは亢進しない

即使在急性热暴露期间，蛋鸡的氧化应激也不会增加

• 发表时间: 2012
• 作者: Amagai Y;Tanaka A;Matsuda A;Oida K;Jung K;Matsuda H;喜久里基・豊水正昭
• 通讯作者: 喜久里基・豊水正昭

急性暑熱感作鶏における酸化損傷の組織特異性

急性热敏鸡氧化损伤的组织特异性

• 发表时间: 2011
• 作者: Sai T;Matsuda F;Goto Y;Maeda A;Sugimoto M;Gao H;Kabir AKMA;Li JY;Manabe N;喜久里基・豊水正昭
• 通讯作者: 喜久里基・豊水正昭

Olive oil (refined)-enriched diet upregulates avian uncoupling protein expression and proton leak in the skeletal muscle mitochondria

富含橄榄油（精制）的饮食上调鸟类骨骼肌线粒体中解偶联蛋白的表达和质子泄漏

• 发表时间: 2012
• 作者: Kikusato;M.;Uwabe;Y. and Toyomizu;M.
• 通讯作者: M.