Research for the effect of uric acid crystal and adenosine triphosphate on skin immunity and skin diseases

尿酸结晶、三磷酸腺苷对皮肤免疫及皮肤病影响的研究

• 批准号: 23890248
• 负责人: KAWASHIMA Tomohiko
• 金额: $ 2.08万
• 依托单位: 公益財団法人冲中記念成人病研究所 (2012)Okinaka Memorial Institute for Medical Research (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23890248/
• 关键词: 皮膚免疫; 尿酸結晶; アデノシン三リン酸; ランゲルハンス細胞; 樹状細胞

We studied the effect of MSU on Langerhans cells (LC). We confirmed that LC has NALP-3 mRNA and ASC protein by RT-PCR and immunoblot. LC were phagocytosing MSU crystals by electron microscopy. Immunoblot of LC cell lysate demonstrated that MSU stimulation yielded active caspase-1. MSU significantly increased the IL-18 production from LC. Our preliminary data suggested that LC cultured with MSU were promoted to migrate out of the skin to regional lymph node by MSU stimulation. Thus, MSU would stimulate skin immune response via promoting LC migration.

本文研究了MSU对郎格罕细胞（LC）的作用。RT-PCR和免疫印迹证实LC中存在NALP-3 mRNA和ASC蛋白。电镜下LC吞噬MSU晶体。LC细胞裂解物的免疫印迹表明，MSU刺激产生活性半胱天冬酶-1。MSU显著增加LC产生IL-18。我们的初步数据表明，MSU刺激促进LC从皮肤迁移到区域淋巴结。因此，MSU可通过促进LC迁移来刺激皮肤免疫应答。