Investigation on coordinate regulation of reproduction and related behaviors by kisspeptin neuronal system

在这项研究中，为了揭示脊椎动物中由kisspeptin神经元调节的新型神经元回路，我用GFP可视化了表达kisspeptin受体的神经元，并对青鳉中的GFP阳性神经元进行了RNAseq。研究表明，与摄食行为和稳态有关的肽能神经元表达kisspeptin受体。这些建议最终证实了GFP和某种神经递质的mRNA的原位杂交双标记。电生理检查显示，kisspeptin合成肽使这些表达kisspeptin受体的神经元去活化。作为肽能神经元，如异催产素和催产素神经元被证明表达kisspeptin受体，通过使用TALEN技术敲除这些递质的青鳉品系。这些神经回路的进一步检查将通过利用产生的敲除和转基因青鳉品系的更详细的电生理学检查。

In this study, to unveil the novel neuronal circuits that are regulated by kisspeptin neurons in vertebrates, I visualized kisspeptin receptor-expressing neurons with GFP, and performed RNAseq of GFP positive neurons in medaka. It was shown that the peptidergic neurons that are suggested to be involved in feeding behavior and homeostasis are expressing kisspeptin receptor. These suggestion were finally confirmed by double labeling of GFP and mRNA of a certain neurotransmitter by in situ hybridization. Following electrophysiological examination showed that kisspepitn synthetic peptide depolarize these kisspeptin receptor expressing neurons. As peptidergic neurons such as isotocin and vasotocin neurons were shown to express kisspeptin receptor, knockout medaka lines of these transmitters by using TALEN technology. Further examination of these neural circuits will be examined by more detailed electrophysiology utilizing the generated knockout and transgenic medaka lines.