Investigation on coordinate regulation of reproduction and related behaviors by kisspeptin neuronal system

在这项研究中，为了揭示脊椎动物中受Kispeptin神经元调控的新的神经元回路，我用GFP显示了Kispeptin受体表达的神经元，并对青竹的GFP阳性神经元进行了RNASEQ。结果表明，参与摄食行为和动态平衡的多肽能神经元表达Kispeptin受体。通过GFP和某一神经递质的mRNA的原位杂交双标，最终证实了上述推测。随后的电生理检查显示，Kispepetn合成肽对这些Kispeptin受体表达的神经元去极化。用TALEN技术敲除这些递质的青山系，发现异托素和血管催产素等多肽能神经元表达Kispeptin受体。对这些神经回路的进一步检查将通过更详细的电生理学进行检查，使用产生的敲除和转基因青竹品系。

In this study, to unveil the novel neuronal circuits that are regulated by kisspeptin neurons in vertebrates, I visualized kisspeptin receptor-expressing neurons with GFP, and performed RNAseq of GFP positive neurons in medaka. It was shown that the peptidergic neurons that are suggested to be involved in feeding behavior and homeostasis are expressing kisspeptin receptor. These suggestion were finally confirmed by double labeling of GFP and mRNA of a certain neurotransmitter by in situ hybridization. Following electrophysiological examination showed that kisspepitn synthetic peptide depolarize these kisspeptin receptor expressing neurons. As peptidergic neurons such as isotocin and vasotocin neurons were shown to express kisspeptin receptor, knockout medaka lines of these transmitters by using TALEN technology. Further examination of these neural circuits will be examined by more detailed electrophysiology utilizing the generated knockout and transgenic medaka lines.