Update of breed improvement system using grafting and phloem transportable RNA.

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GrIGS (Graft induced gene silencing) system is the epigenome editing induction method to a graft partner via long-distance transportable promoter-targeting siRNA through the phloem. The application of this system is one of the new plant breeding techniques (NBT) to improve the cultivars of various horticultural crops. Since the produced plants do not contain any transgenes, these do not correspond to GM plants. In this study, I examined the improvement of this system and its application. As a result, epigenome editing was induced efficiently by including transient expression assay of the transportable signals and signal amplification system, furthermore also in the endogenous gene promoter of tomato.