NBT REDUCTION TEST, VITAMIN A・E AND MASTITIS

NBT 还原测试、维生素 A・E 和乳腺炎

• 批准号: 03660325
• 负责人: HAMANA Katsumi
• 金额: $ 0.58万
• 依托单位: KAGOSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660325/
• 关键词: Dairy Cattle; NBT Reduction Test,; Vitamin A; Vitamin E; Mastitis,; Teat; Ultrasonography; 好中球; Retinol; αーTocopherol

In this research, effects of vitamin A (Retinol) and vitamin E (alphatocopherol) on subclinical mastitis and on bactericidal ability of neutrophils were investigated. In addition, teats which is the most important factor in mastitis were investigated morphologically.Forty dairy cattle kept in a good feeding and management farm were monthly examined on milk yield and quality, mastitis tests, blood analysis, serum vitamin A and E level for one year. Electronic conductivity and somatic cell counts in milk were found to have a relatively well correlation to milk yield and quality. Serum vitamin E levels was found to have some relation to these test results.Effect of retinol and alpha-tocopherol was investigated in vitro on the bactericidal ability of neutrophils obtained from 20 lactating cows. Nitroblue tetrazolium (NBT) reduction test was employed. After the isolation of neutrophils from blood, NBT reduction test was conducted under the condition of intact, retinol and alpha-tocopherol a … More ddition. The most high NBT reduction ability was obtained at 100 IU/dl of retinol and at 200 mug/dl of alpha-tocopherol, respectivily. Ethanol, retinol or alpha-tocopherol were added to the medium of neutrophil's culture. Though NBT reduction ability decreased to about half level in control, it was well preserved in retinol or alpha-tocopherol addition. As a result, it was suggested that the bactericidal ability of neutrophils from lactating cows was influenced in vitro by the level of retinol or alpha-tocopherol, and was well preserved under the presence of retinol or alpha-tocopherol.Several parts of the teats were measured and its results were compared to milk quality and bacteria. It was found that the teat with bigger teat gradient or teat canal diameter is liable to mastitis. Then, the teats were examined by ultrasonography, macroscopically and histopathologically. Abnormal findings were diagnosed by ultrasonography as follows, obstraction of teat sinus, hypertrophy of intima, abscess, polyp, diverticulum, stenosis and malformation. They were confirmed by morphological examination. Less

在这项研究中，维生素A（视黄醇）和维生素E（维生素E）对隐性乳腺炎和中性粒细胞杀菌能力的影响进行了研究。此外，还对影响乳房炎的重要因素--乳头进行了形态学研究，并对40头奶牛进行了为期一年的产奶量、乳品质、乳房炎试验、血液分析、血清维生素A和E水平的每月检查。电导率和体细胞数与产奶量和品质有较好的相关性。血清维生素E水平与这些测试结果有一定关系。体外研究了视黄醇和α-生育酚对20头泌乳奶牛中性粒细胞杀菌能力的影响。采用硝基四氮唑蓝（NBT）还原试验。从血液中分离中性粒细胞后，在完整、维生素A和α-生育酚A的条件下进行NBT还原试验， ...更多信息 ddition。最高的NBT还原能力分别在100 IU/dl的视黄醇和200 mug/dl的α-生育酚时获得。中性粒细胞培养液中加入乙醇、视黄醇或α-生育酚。虽然NBT还原能力下降到一半左右的水平，在对照组中，它是很好地保存在视黄醇或α-生育酚添加。结果表明，泌乳奶牛中性粒细胞在体外杀菌能力受视黄醇或α-生育酚水平的影响，在视黄醇或α-生育酚存在下，中性粒细胞的杀菌能力保持良好。结果表明，乳头倾斜度大或乳头管直径大的乳头易患乳腺炎。然后，对乳头进行超声检查、大体观察和组织病理学检查。超声诊断乳头窦阻塞、内膜肥厚、脓肿、息肉、憩室、狭窄、畸形。通过形态学检查证实。少

项目成果

Motomura, Y., Yasuda, N., Kamimura, S. and Hamana, K.: "Ultrasonic tomography and morphological observation on normal and abnormal teat of dairy cattle." J. Jpn. Vet. Med. Assoc.(1993)

Motomura, Y.、Yasuda, N.、Kamimura, S. 和 Hamana, K.：“奶牛正常和异常乳头的超声断层扫描和形态学观察”。

Hamana, K., Kudo, Y. and Taura, Y.: "Effects of subclinical mastis on milk components and serum vitamin A and E." The Bulletin of the Faculty of Agriculture, Kagoshima University. 43. 49-57 (1993)

Hamana, K.、Kudo, Y. 和 Taura, Y.：“亚临床乳腺炎对乳成分和血清维生素 A 和 E 的影响”。

元村 泰彦,安田 宣紘,上村 俊一,浜名 克己: "乳牛の正常および異常乳頭の超音波診断と形態学的観察" 日本獣医師会雑誌. (1993)

Yasuhiko Motomura、Nobuhiro Yasuda、Shunichi Uemura、Katsumi Hamana：“奶牛正常和异常乳头的超声诊断和形态学观察”日本兽医协会杂志（1993）。

元村 泰彦,安田 宣紘 上村 俊一,浜名 克己: "乳牛の正常および異常乳頭の超音波診断と形態学的観察" 日本獣医師会雑誌. (1993)

Yasuhiko Motomura、Nobuhiro Yasuda、Shunichi Uemura、Katsumi Hamana：“奶牛正常和异常乳头的超声诊断和形态学观察”日本兽医协会杂志（1993）。

元村 泰彦,抽木 弘之,上村 俊一,浜名 克己: "乳牛の乳頭測定値と乳質、細菌検査の関連" 日本獣医師会雑誌. (1993)

Yasuhiko Motomura、Hiroyuki Aki、Shunichi Uemura、Katsumi Hamana：“奶牛乳头测量、牛奶质量和细菌检测之间的关系”日本兽医协会杂志（1993 年）。