NBT REDUCTION TEST, VITAMIN A・E AND MASTITIS

本研究探讨维生素A(视黄醇)和维生素E(甲酚)对亚临床型乳房炎的治疗作用及对中性粒细胞杀菌能力的影响。对40头饲养管理较好的奶牛场饲养的40头奶牛进行了为期一年的月度产奶量和品质、乳房炎试验、血液分析、血清维生素A、E水平的检测。研究发现，牛奶中的电导率和体细胞数与牛奶的产量和品质有较好的相关性。研究了维生素E和α-生育酚对20头奶牛中性粒细胞体外杀菌能力的影响。采用四氮基偶氮唑蓝还原试验。从血液中分离中性粒细胞后，在完整、视黄醇和α-生育酚a…的条件下进行nbt还原试验。更多的离奇。当视黄醇为100IU/dl、α-生育酚为200ug/dl时，NBT还原能力最强。在中性粒细胞培养上清液中加入乙醇、视黄醇或α-生育酚。虽然NBT还原能力在对照组下降到大约一半的水平，但在添加视黄醇或α-生育酚时，它被很好地保存下来。结果表明，泌乳奶牛中性粒细胞体外杀菌能力受视黄醇或α-生育酚水平的影响，在视黄醇或α-生育酚存在下，中性粒细胞杀菌能力较好。研究发现，乳头倾斜度或乳头管径较大者易患乳房炎。然后对乳头进行大体及组织病理学的超声检查。超声诊断异常表现为乳头窦闭塞、内膜肥大、脓肿、息肉、憩室、狭窄及畸形。经形态检查证实。较少

In this research, effects of vitamin A (Retinol) and vitamin E (alphatocopherol) on subclinical mastitis and on bactericidal ability of neutrophils were investigated. In addition, teats which is the most important factor in mastitis were investigated morphologically.Forty dairy cattle kept in a good feeding and management farm were monthly examined on milk yield and quality, mastitis tests, blood analysis, serum vitamin A and E level for one year. Electronic conductivity and somatic cell counts in milk were found to have a relatively well correlation to milk yield and quality. Serum vitamin E levels was found to have some relation to these test results.Effect of retinol and alpha-tocopherol was investigated in vitro on the bactericidal ability of neutrophils obtained from 20 lactating cows. Nitroblue tetrazolium (NBT) reduction test was employed. After the isolation of neutrophils from blood, NBT reduction test was conducted under the condition of intact, retinol and alpha-tocopherol a … More ddition. The most high NBT reduction ability was obtained at 100 IU/dl of retinol and at 200 mug/dl of alpha-tocopherol, respectivily. Ethanol, retinol or alpha-tocopherol were added to the medium of neutrophil's culture. Though NBT reduction ability decreased to about half level in control, it was well preserved in retinol or alpha-tocopherol addition. As a result, it was suggested that the bactericidal ability of neutrophils from lactating cows was influenced in vitro by the level of retinol or alpha-tocopherol, and was well preserved under the presence of retinol or alpha-tocopherol.Several parts of the teats were measured and its results were compared to milk quality and bacteria. It was found that the teat with bigger teat gradient or teat canal diameter is liable to mastitis. Then, the teats were examined by ultrasonography, macroscopically and histopathologically. Abnormal findings were diagnosed by ultrasonography as follows, obstraction of teat sinus, hypertrophy of intima, abscess, polyp, diverticulum, stenosis and malformation. They were confirmed by morphological examination. Less