New approaches to understanding the regulation of ER-subcompartment dynamics by using experimental techniques in endocrinology.

利用内分泌学实验技术了解 ER 亚室动态调节的新方法。

• 批准号: 24890012
• 负责人: BOCHIMOTO Hiroki
• 金额: $ 1.91万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-08-31 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24890012/
• 关键词: 実験内分泌学的手法; 電子顕微鏡; 小胞体; 小胞体サブコンパートメント; 小胞体関連分解; 内分泌細胞; 下垂体前葉; 性腺刺激ホルモン産生細胞

To examine the morphological and functional characteristics of the ER in vivo, we analyzed the ER changes of male rat pituitary gonadotropes during sustained treatment with various GnRH analogs. In pituitary gonadotropes at 1 day after subcutaneous implantation of osmotic pumps filled with a GnRH agonist, leuprolide acetate, patch-like accumulations of two representative chaperons, calnexin and BiP appeared. Similar changes were observed under the condition of another GnRH agonist, buserelin. Observation at the electron microscopic level could reveal atypical accumulations of the ER chaperones in anomalous clusters of tubuloreticular membranes within the stimulated gonadotrope. In addition to the chaperones, HRD1, an E3 ligase related to ER associated degradation (ERAD), is localized to the ER patches and surrounding membranes in the stimulated gonadotropes. These findings indicate the anomalous ER clusters provide a scaffold for quality control of secretion proteins in gonadotropes.

为了研究ER在体内的形态和功能特征，我们分析了雄性大鼠垂体促性腺激素细胞在持续使用各种GnRH类似物治疗期间ER的变化。在垂体促性腺激素中，皮下植入装有GnRH激动剂醋酸亮丙瑞林的渗透泵后1天，出现了两种代表性伴侣蛋白钙连接蛋白和BiP的斑块样蓄积。在另一种GnRH激动剂布舍瑞林的条件下观察到类似的变化。在电子显微镜水平上的观察可以揭示非典型的聚集的ER分子伴侣在异常集群的tubuloreticular膜内的刺激促性腺细胞。除了分子伴侣，HRD 1，一种与ER相关降解（ERAD）相关的E3连接酶，定位于刺激的促性腺激素细胞中的ER斑和周围膜。这些发现表明异常ER簇为促性腺激素分泌蛋白的质量控制提供了一个支架。

项目成果

研究者詳細 - 暮地本 宙己

研究员详情 - Hiroki Kurejimoto

Functional Implications of the Golgi and Microtubular Network in Gonadotropes

高尔基体和微管网络在促性腺激素中的功能意义

• DOI: 10.1016/j.mce.2013.10.003
• 发表时间: 2014
• 期刊: Molecular and Cellular Endocrinology
• 影响因子: 4.1
• 作者: ②Watanabe T;Bochimoto H;Koga D;Hosaka M;Ushiki T
• 通讯作者: Ushiki T

GnRHレセプター下流のシグナル伝達がラット下垂体前葉LH/FSH産生細胞の粗面小胞体に与える影響

GnRH受体下游信号对大鼠垂体前叶LH/FSH产生细胞粗面内质网的影响

• 发表时间: 2013
• 作者: 暮地本宙己;穂坂正博;板東良雄;甲賀大輔;平義樹;牛木辰男;渡部剛
• 通讯作者: 渡部剛

Sustained treatment with a GnRH agonist (leuprorelin) affects the ultrastructural characteristics of endomembranous organelles in male rat pituitary gonadotropes.

GnRH 激动剂（亮丙瑞林）持续治疗会影响雄性大鼠垂体促性腺激素内膜细胞器的超微结构特征。

• 发表时间: 2013
• 期刊: Archives of Histology and Cytology
• 作者: Bochimoto H;Koga D;Sakai Y;Hira Y;Hosaka M;Ushiki T;Watanabe T
• 通讯作者: Watanabe T

Focal adhesion kinase regulates intestinal epithelial barrier function via redistribution of tight junction

• DOI: 10.1016/j.bbadis.2012.10.006
• 发表时间: 2013-01-01
• 期刊: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
• 影响因子: 6.2
• 作者: Ma, Yanju;Semba, Shingo;Taniguchi, Takanobu
• 通讯作者: Taniguchi, Takanobu