Analysis of miRNA in the liver cancer stem cell: For cancer chemoprevention and metastasis suppression

肝癌干细胞中的 miRNA 分析：用于癌症化学预防和转移抑制

• 批准号: 24890081
• 负责人: SUETSUGU Atushi
• 金额: $ 1.91万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-08-31 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24890081/
• 关键词: 肝癌; 癌幹細胞; miRNA

Cancer cells form microenvironment in primary tumor and metastatic formation. The cancer-associated cells play an important role. Cancer-associated fibroblast cells and tumor-associated macrophages are important at the time of the liver metastasis formation. Cancer cells shed exosomes and transfer exosomal molecules to fibroblast cells during tumor progression. We confirmed that cancer cells release exosomes into the circulation of mice with liver metastasis. We collected blood from mice and detected exosomes. Imaging of exosomes can contribute to understanding the role of intercellular communication in tumor progression. Tumor-drived exosomes have been viewd as a mediater to condition tissues and prepare niches for metastasis.

癌细胞在原发肿瘤和转移形成过程中形成微环境。癌症相关细胞起着重要的作用。肿瘤相关成纤维细胞和肿瘤相关巨噬细胞在肝转移形成过程中起重要作用。在肿瘤进展过程中，癌细胞脱落外泌体并将外泌体分子转移到成纤维细胞。我们证实癌细胞将外泌体释放到肝转移小鼠的循环中。我们从小鼠身上采集血液并检测外泌体。外泌体成像有助于理解细胞间通讯在肿瘤进展中的作用。肿瘤驱动的外泌体被认为是调节组织和为转移准备龛的介质。

项目成果

Cancer cell location in tumor determines cell cycle position and dormancy

肿瘤中癌细胞的位置决定细胞周期位置和休眠

• 发表时间: 2013
• 作者: Yano S;Tome Y;Michelle Digman;Momiyama M;Suetsugu A;Enrico Gratton;Hoffman RM
• 通讯作者: Hoffman RM

シンポジウム C型慢性肝炎IFN治療SVR後の発癌症例の検討

慢性丙型肝炎 IFN 治疗 SVR 后癌症病例的研讨会检查

• 发表时间: 2013
• 作者: 末次 淳;内木隆文;森脇久隆
• 通讯作者: 森脇久隆

Treatment of brain tumors in nude mice with intra-cranial UV irradiation

颅内紫外线照射治疗裸鼠脑肿瘤

• 发表时间: 2012
• 作者: Satoshi Hasegawa;Momiyama M;Hiroshima Y;Suetsugu A;Tome Y;Mii S;Syuya Yano;Chishima T;Micheal Bouvet;Hoffman RM
• 通讯作者: Hoffman RM

Paxillin-expressing breast cancer imaged by raster-image-correlation spectroscopy of GFP in vivo and in vitro

通过体内和体外 GFP 光栅图像相关光谱对表达桩蛋白的乳腺癌进行成像

• 发表时间: 2012
• 作者: Suetsugu A;Michelle Digman;Federica Sabatini;Moriwaki H;Saji S;Micheal Bouvet;Enrico Grattion;Hoffman RM
• 通讯作者: Hoffman RM

Making patient tumors glow in nude mice by coloring the stroma with fluorescent proteins

通过用荧光蛋白对基质进行着色，使裸鼠体内的患者肿瘤发光

• 发表时间: 2012
• 作者: Suetsugu A;Katz MH;Fleming J;Mark Truty;Saji S;Moriwaki H;Bouvet M;Hoffman RM
• 通讯作者: Hoffman RM