Mouse experimental myopia model and cell-type specific NGS profiling

近视在日本是一种常见的眼部疾病，其患病率似乎超过40%。高度近视，又称病理性近视，是东北亚国家威胁视力的主要疾病之一。 有研究表明，近视眼的神经信号来源于视网膜内层，但目前尚不清楚哪种细胞类型对近视信号的贡献最大。在这项研究中，小鼠神经节细胞分离和富集与流式细胞术（FACS），并提取的RNA进行优化的RNA测序，进行下一代测序仪（NGS）。该方法可应用于实验性近视小鼠，以揭示近视的病理机制。

Myopia is a common ocular status in Japanese population, whose prevalence seems to be more than 40%. High myopia, also known as pathologic myopia, is one of the top vision threatening diseases in Northeastern Asian countries. It was reported that the neural signal enhancing myopia is originated at the inner layer of retina, but it is not clear which cell type has a largest contribution for the myopic signal. In this study, mouse ganglion cell was dissociated and enriched with flow-cytometry (FACS), and the extracted RNA was optimized for RNA Seq, performed with next generation sequencer (NGS). This approach can be applied for experimental myopic mice for revealing the pathomechanism of myopia.