Mouse experimental myopia model and cell-type specific NGS profiling

小鼠实验性近视模型和细胞类型特异性 NGS 分析

• 批准号: 24890100
• 负责人: GOTOH NORIMOTO
• 金额: $ 1.91万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-08-31 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24890100/
• 关键词: 眼細胞生物学; 眼生化学・分子生物学; 眼遺伝学; 眼科; 神経科学; ゲノム医学; 国際情報交換（アメリカ）; 国際研究交流（アメリカ）

Myopia is a common ocular status in Japanese population, whose prevalence seems to be more than 40%. High myopia, also known as pathologic myopia, is one of the top vision threatening diseases in Northeastern Asian countries. It was reported that the neural signal enhancing myopia is originated at the inner layer of retina, but it is not clear which cell type has a largest contribution for the myopic signal. In this study, mouse ganglion cell was dissociated and enriched with flow-cytometry (FACS), and the extracted RNA was optimized for RNA Seq, performed with next generation sequencer (NGS). This approach can be applied for experimental myopic mice for revealing the pathomechanism of myopia.

近视是日本人群中常见的眼部疾病，患病率似乎超过40%。高度近视，又称病理性近视，是东北亚国家威胁视力的首要疾病之一。 据报道，增强近视的神经信号起源于视网膜内层，但尚不清楚哪种细胞类型对近视信号的贡献最大。在本研究中，使用流式细胞仪 (FACS) 分离并富集小鼠神经节细胞，并使用下一代测序仪 (NGS) 进行 RNA 测序，对提取的 RNA 进行优化。该方法可应用于实验性近视小鼠，揭示近视的病理机制。

项目成果

Association Between ZIC2, RASGRF1, and SHISA6 Genes and High Myopia in Japanese Subjects

• DOI: 10.1167/iovs.13-12825
• 发表时间: 2013-11-01
• 期刊: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
• 影响因子: 4.4
• 作者: Oishi, Maho;Yamashiro, Kenji;Yoshimura, Nagahisa
• 通讯作者: Yoshimura, Nagahisa

Identification of the WNT7B gene provides the mechanism underlying myopia development

WNT7B基因的鉴定提供了近视发展的机制

• 作者: Miyake M;Yamashiro K;Tabara Y;Suda K;Morooka S;Nakanishi H;Khor CC;Chen P;Nakata I;Akagi-Kurashige Y;Gotoh N;Tsujikawa A;the Nagahama Study Group;Meguro A;Kusuhara S;Polasek O;Hayward C;Wright AF;Campbell H;Richardson AJ;Schache M;Tak
• 通讯作者: Tak