ion transport and regulation of the ion transport in the inner ear and endolymphatic sac

内耳和内淋巴囊中的离子运输和离子运输的调节

• 批准号: 24890155
• 负责人: MIYASHITA Takenori
• 金额: $ 2万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-08-31 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24890155/
• 关键词: 内リンパ嚢; 内耳; イオン輸送; イオンチャネル; メニエール病; イオンイメージング; 内リンパ調節; 内リンパ水腫; 内耳生理; 耳科学; イメージング

We demonstrated the expression of Prox1 in all parts of the endolymphatic sac epithelia in adult stage. Prox1 was used as a marker for the endolymphatic sac epithelia. The use of Prox1-GFP mice made possible the whole-mount imaging and three-dimensional observations of the endolymphatic sac epithelial cells through decalcified temporal bone. Using this whole-mount imaging technique and vascular staining with dextran-rhodamine, the endolymphatic sac was observed. In the proximal portion, the endolymphatic sac was narrow and surrounded by few blood vessels. In the intermediate portion, which is the most active ion transporting part, the endolymphatic sac protruded and was surrounded by many branched blood vessels. In the distal portion, the endolymphatic sac was close to the sigmoid sinus. The same samples were sectioned and stained by immunohistochemistry. This whole-mount imaging technique of the endolymphatic sac will be a very useful method for endolymphatic sac research.

在成体阶段，Prox1在内淋巴囊上皮细胞的各个部位均有表达。PROX1作为内淋巴囊上皮细胞的标志物。Prox1-GFP小鼠的使用使得通过脱钙的颞骨对内淋巴囊上皮细胞进行整体成像和三维观察成为可能。应用这种整体成像技术和右旋糖苷-罗丹明血管染色技术，观察内淋巴囊。近端内淋巴囊狭窄，周围血管稀少。在离子转运最活跃的中间部分，内淋巴囊突出，周围环绕着许多分支血管。在远端，内淋巴囊靠近乙状窦。对同一标本进行切片和免疫组织化学染色。这种内淋巴囊的整体成像技术将是一种非常有用的内淋巴囊研究方法。

项目成果

Evaluation of the vestibular aqueduct in Meniere's disease using multiplanar reconstruction images of CT

• DOI: 10.1016/j.anl.2011.11.005
• 发表时间: 2012-12-01
• 期刊: AURIS NASUS LARYNX
• 影响因子: 1.7
• 作者: Miyashita, Takenori;Toyama, Yoshihiro;Mori, Nozomu
• 通讯作者: Mori, Nozomu

内リンパ嚢におけるPannexin1の発現

Pannexin1 在内淋巴囊中的表达

• 发表时间: 2013
• 作者: 宮下武憲;森望
• 通讯作者: 森望

Localization and proliferation of lymphatic vessels in the tympanic membrane in normal state and regeneration.

正常状态下鼓膜内淋巴管的定位和增殖及再生。

• DOI: 10.1016/j.bbrc.2013.09.054
• 发表时间: 2013
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Miyashita,Takenori;Burford,JamesL;Hong,Young-Kwon;Gevorgyan,Haykanush;Lam,Lisa;Mori,Nozomu;Peti-Peterdi,Janos
• 通讯作者: Peti-Peterdi,Janos

マウス内リンパ嚢におけるProx１の発現

Prox1在小鼠内淋巴囊中的表达

• 发表时间: 2013
• 作者: 宮下武憲;森 望
• 通讯作者: 森 望

ラット内リンパ嚢におけるcystic fibrosis transmembrane conductance regulator (CFTR)の発現

囊性纤维化跨膜电导调节因子（CFTR）在大鼠内淋巴囊中的表达

• 发表时间: 2013
• 作者: 松原あい;宮下武憲;稲本隆平;星川広史;森望
• 通讯作者: 森望