Structural analyses of inflammatory HMGB1-IL1beta complex

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Basically HMGB1 plays a role in gene transcription in nucleus. However, leaked HMGB1 out of the cells induce inflammation around the cell. The inflammatory reaction can be augmented by interacting with IL-1beta, which is one of the potent inflammatory cytokines.Recombinant HMGB1 and IL-1beta proteins could be sucsessfully over-expressed by using Escherichia coli expression system. Then, we could highly purified the recombinant HMGB1 and IL-1beta proteins by performing several types of chromatographic experiments. Furthermore, binding affinity between the recombinant HMGB1 and IL-1beta proteins were investigated by measuring fluorescence depolarization.