Stoffwechsel-Käfigsystem

代谢笼系统

• 批准号: 454765876
• 金额: --
• 依托单位: Universität Hamburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/454765876?language=en
• 关键词: Stoffwechsel; figsystem

Metabolic disturbances are causally involved in the development of chronic inflammatory diseases such as atherosclerosis, diabetes, non-alcoholic steatohepatitis (NASH), inflammatory diseases of the intestines and bones and various tumours. Current epidemiological studies show that more than half of the world population is now overweight, with the high number of obese children being a particular cause for concern. The economic consequences of this development are not yet foreseeable, so that new science-based strategies for the treatment of obesity-related diseases are urgently needed. With a positive energy balance, excess calories are stored as lipids in the white fat tissue, while the energy-consuming brown and beige fat cells form the so-called thermogenic fat tissue. This is activated in cold environments to protect humans and other mammals from a drop in body temperature. In addition, thermogenic fat cells have the potential to counteract obesity and metabolic diseases. The activity of these thermogenic fat cells, also in connection with immune cell activity, the processing of food in the intestine, exercise and regulation by the nervous system, can be determined by indirect calorimetry. Using this non-invasive method, metabolic activity can be determined by the very accurate measurement of oxygen consumption and carbon dioxide production, body temperature and activity in the day and night profile at different ambient temperatures in mice. New systems for the determination of these parameters enable a significantly higher sensitivity and resolution, so that changes in these elementary metabolic parameters can also be detected, e.g. in inflammatory changes or diseases of the skeletal system. The proposed system will thus enable cross-organ systematic analyses at the interface between metabolism and inflammation in order to better understand the significance of metabolic changes at different ambient temperatures and diets for the development of chronic inflammatory diseases in the fatty tissue, liver, bones, central nervous system and intestine. The studies are conducted by four different research groups within the framework of DFG-funded individual projects and collaborative research centres at the University Medical Center Hamburg-Eppendorf (UKE). The system for indirect calorimetry would thus support the designated UKE research focus on Immunology, Immunity, Infection (C3i). It is planned that the applicant will also offer the technology to other research groups as a core facility unit.

代谢紊乱与慢性炎症性疾病如动脉粥样硬化、糖尿病、非酒精性脂肪性肝炎（NASH）、肠道和骨骼的炎症性疾病以及各种肿瘤的发展有因果关系。目前的流行病学研究表明，现在世界上一半以上的人口超重，肥胖儿童人数之多尤其令人担忧。这种发展的经济后果还无法预见，因此迫切需要新的基于科学的治疗肥胖相关疾病的策略。在正能量平衡状态下，多余的卡路里以脂质形式储存在白色脂肪组织中，而消耗能量的棕色和米色脂肪细胞形成所谓的产热脂肪组织。它在寒冷的环境中被激活，以保护人类和其他哺乳动物免受体温下降的影响。此外，产热脂肪细胞具有对抗肥胖和代谢疾病的潜力。这些产热脂肪细胞的活动，也与免疫细胞活动、肠道食物加工、运动和神经系统调节有关，可通过间接量热法测定。使用这种非侵入性方法，代谢活动可以通过非常精确地测量氧气消耗和二氧化碳产生，体温和活动在不同环境温度下的小鼠白天和黑夜的轮廓来确定。用于测定这些参数的新系统具有更高的灵敏度和分辨率，因此也可以检测到这些基本代谢参数的变化，例如在炎症变化或骨骼系统疾病中。因此，所提出的系统将能够在代谢和炎症之间的界面上进行跨器官系统分析，以便更好地了解不同环境温度和饮食下代谢变化对脂肪组织、肝脏、骨骼、中枢神经系统和肠道慢性炎症疾病发展的意义。这些研究由四个不同的研究小组在dfg资助的个人项目和汉堡-埃彭多夫大学医学中心（UKE）的合作研究中心的框架内进行。因此，间接量热系统将支持英国大学指定的免疫学、免疫、感染（C3i）研究重点。计划申请人还将把该技术作为核心设施单元提供给其他研究小组。