Differential chromatin packaging of genomic imprinted regions between expressed and non-expressed alleles

表达和非表达等位基因之间基因组印记区域的差异染色质包装

• 批准号: 11470033
• 负责人: KOMINAMI Ryo
• 金额: $ 9.54万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470033/
• 关键词: genomic imprinting; chromatin packaging; allele-specific; blueprints; ゲノムインプリント; 内部欠失; 欠失領域; クロマチン凝縮度; 臓器特異的発見; コンジェニックマウス; クロマチン分画法; 多型; アレル

Chromosomal regions subject to genomic imprinting comprise a functional domain exhibiting parental-specific expression of genes and hence may take a Unique chromatin structure. Here we have examined chromatin packaging state of allelic sites in the Zfpl27/Snrpn locus on mouse chromosome 7 and in the Igf2r locus on mouse chromosome 17 with an assay consisting of chromatin fractionation and allele-specific detection. The result showed that nontranscribed alleles of IgfSr are packaged more compact than transcribed alleles in F_1 hybrid mice of both types of cross between C57BL/6 and MSM strains, while a nonimprinted gene, Sod-2, in the vicinity of Igf2r does not show such a difference. This indicates a close correlation between imprinting and the differential packaging of chromatin. On the other hand, the Zfpl27/Snrpn locus showed such an allele-specific fractionation pattern only in Ft hybrid mice of a cross but not in those of the reciprocal cross.Analysis of congenic mice produced for this locus did not provide any difference. These results suggest the chromatin of imprinted domains in different compaction level which is affected by distinct blueprints in homologous chromosomes that are heritable through the germ line.

受到基因组印记的染色体区域包含表现出亲本特异性基因表达的功能域，因此可能具有独特的染色质结构。在这里，我们通过由染色质分级和等位基因特异性检测组成的测定法检查了小鼠 7 号染色体上的 Zfpl27/Snrpn 基因座和小鼠 17 号染色体上的 Igf2r 基因座等位基因位点的染色质包装状态。结果表明，C57BL/6和MSM品系杂交的两种类型的F_1杂交小鼠中，IgfSr的非转录等位基因比转录等位基因包装得更紧凑，而Igf2r附近的非印迹基因Sod-2则没有表现出这种差异。这表明印记与染色质的差异包装之间存在密切相关性。另一方面，Zfpl27/Snrpn基因座仅在杂交的Ft杂种小鼠中显示出这种等位基因特异性分级模式，而在互交的那些小鼠中则没有。对该基因座产生的同源小鼠的分析没有提供任何差异。这些结果表明，不同压实水平的印记域的染色质受到可通过种系遗传的同源染色体中不同蓝图的影响。

项目成果

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T. Shinbo 等人：“小鼠 12 号染色体上含有推定胸腺淋巴瘤抑制基因的区域的等位基因丢失图谱和物理描绘。”癌基因。

T.Watanabe: "Differential chromatin packaging of genomic imprinted regions between expressed and non-expressed alleles"Human Molecular Genetics. 9. 3029-3035 (2000)

T.Watanabe：“表达和非表达等位基因之间基因组印记区域的差异染色质包装”人类分子遗传学。

A.Yoshimura: "Mononucleosomes assembled on a DNA fragment containing (GGA/TCC)n repeats can form a DNA-DNA complex"Biochemical Biophysical Research Communications. 290. 16-22 (2002)

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