Endocrinological analysis of polycystic ovary syndrome (PCO)

为了研究多囊卵巢综合征（PCO）的潜在机制，我们使用了一种正常排卵率低于4的Hartley品系豚鼠。鉴于目前豚鼠超数排卵的诱导困难，Hartley品系雌性豚鼠将成为通过刺激卵泡生成来研究PCO内分泌机制的良好模型。1）雌性豚鼠自发性卵巢囊肿的研究：在雌性豚鼠的发情周期中观察到三种类型的卵巢囊肿。64%的动物出现浆液性囊肿. 22%的动物出现滤泡性囊肿，常伴有浆液性囊肿.免疫组化分析显示三种类型的卵巢囊肿均无内分泌活性。免疫中和法诱导卵泡发育和超数排卵：给发情周期与孕激素同步的雌性豚鼠注射抗α-卵泡素血清，刺激卵泡发育和雌激素分泌。但此处理并不能增加卵泡中含α-卵泡素亚基的卵泡数和排卵率。另一方面，主动免疫使具有卵泡素α和黄体的卵泡数量增加，平均为10个。为探讨抗人胚白蛋白α免疫豚鼠后胚胎的正常发育能力，将抗人胚白蛋白α免疫的雌性豚鼠与雄性豚鼠交配。体外培养结果表明，回收胚胎发育正常，抑制素α可能参与卵巢囊肿的形成。

To investigate the mechanisms underlying polycystic ovarian syndrome (PCO), we used a Hartley strain of guinea pigs, whose normal ovulation rate is less than four. Since it has been reported that the induction of superovulation in guinea pig is difficult, the female guinea pig of Hartley strain will be a good model to investigate endocrinological mechanisms underlying PCO by stimulating folliculogenesis.1) Investigation of spontaneous ovarian cysts in female guinea pigs : Three types of ovarian cysts were observed during estrous cycle of female guinea pigs. Serous cyst were found in 64 % of animals. Follicular cyst were found in 22 % of animals, always accompanied by serous cysts. Parovarian cyst was rarely found. Immunohistochemical analysis revealed all three types of ovarian cysts were endocrinologically inactive.) Stimulation of follicular development and induction of superovulation by immnoneutralization of inhibin : Injection of anti-inhibin α serum to female guinea pigs, whose estrous cycle were synchronized with progesterone administration, stimulate folliculogenesis and estrogen secretion. However both the number of follicles with inhibin α subunit and the ovulation rate did not increase by this treatment. On the other hand, active immunization with inhibin α subunit resulted in increased numbers of follicles with inhibin α and corpora lutea with average of ten. To investigate the ability of normal development of embryos, female immunized against inhibin α were mated with male guinea pigs. In vitro culture of recovered embryos revealed normal development comparable to control animals.Inhibin α may be involved in the mechanisms of developing cysts in the ovary.