Construction of database on characteristics of genetic resources of orange subfamily plants.

橙亚科植物遗传资源特征数据库的构建

• 批准号: 10460017
• 负责人: NITO Nobumasa
• 金额: $ 6.85万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10460017/
• 关键词: Citrus; Orange subfamily plants; Genetic resources; Database; Phylogeny; Chemotaxonomy; Rootstock; ミカン亜科; 化学分類; リカルシトラント種子; 細胞自己認識; フラボノイド

Genetic resources of 47 species in 22 genera of orange subfamily plants are maintained at Saga University. These plant materials have been evaluated for further uses of the cultivar and rootstock improvement. In the present project, the information on the characteristics of genetic resources are recorded and evaluated to construct the database, which can be linked with other databases on genetic resources.Information on the morphology of flower, fruit, leaf and stomata are recorded and accumulated as a database. The information on anatomical characteristics, components of essential oils and isozyme analysis of 10 strains of Citrus depressa and 7 strains of Poncirus trifoliate are recorded for the phylogenetic study. The dwarfism of rootstock is related to the ratio of bark and xylem. The dwarfism of scion cultivars cannot be estimated by the anatomical research on rootstocks.The analysis of flavonoid compounds in the leaf and organic acids in fruit juice by high pressure liquid chromatography are useful for the chemotaxonomy of orange subfamily plants.

佐贺大学保存有橙子亚科植物22属47种的遗传资源。这些植物材料已被评估用于栽培品种和砧木改良的进一步用途。在本项目中，记录和评价有关遗传资源特征的信息，以建立数据库，该数据库可与其他有关遗传资源的数据库连接，并记录和积累有关花、果、叶和气孔的形态信息，作为一个数据库。对10个川陈皮品系和7个枳品系的解剖学特征、精油成分和同工酶进行了分析，为系统发育研究奠定了基础。砧木的矮化性与皮木比有关。砧木的解剖学研究不能反映接穗品种的矮化性，用高压液相色谱法分析叶片中的黄酮类化合物和果汁中的有机酸，可为橙子亚科植物的化学分类提供参考。

项目成果

Rahman,M.M.,N.Nito and S.Isshiki: "Cultivar identifiction of 'Yuzu' (Citrus junos Sieb.ex Tanaka) and related acid citrus by leaf isozymes."Scientia Horticulturae. 87・. 191-198 (2001)

Rahman，M.M.，N. Nito 和 S. Isshiki：“通过叶同工酶鉴定‘柚子’（Citrus junos Sieb.ex Tanaka）和相关酸性柑橘。”Scientia Horticulturae 87·191-198（2001）。

仁藤伸昌,片山幸良: "ライフサイエンスのための系統保存とデータベース.分担執筆:カンキツ類の系統保存.p.68-73."中辻憲夫 編.共立出版社. 161 (2000)

Nobumasa Nito，Yukiyoshi Katayama：“生命科学的系统保护和数据库。贡献者：柑橘的系统保护。第 68-73 页。共立出版社编辑 161。”

仁藤伸昌,片山幸良: "ライフサイエンスのための系統保存とデータベース.分担執筆:カンキツ類の系統保存"中辻憲夫 編.共立出版社. 161(68-73) (2000)

Nobumasa Nito、Yukiyoshi Katayama：“生命科学的系统保护和数据库。贡献者：柑橘的系统保护”，共立出版社编辑 161(68-73) (2000)。

Rahman, M.M., N.Nito and S.Isshiki: "Cultivar identification of 'Yuzu' (Citrus junos Sieb.ex and related acid citrus by leaf isozymes"Scientia Horticulturae. 87. 191-198 (2001)

Rahman, M.M., N.Nito 和 S.Isshiki：“‘柚子’的品种鉴定（Citrus junos Sieb.ex 和通过叶同工酶相关的酸性柑橘”Scientia Horticulturae. 87. 191-198 (2001)

Nito, N.and Y.Katayama: "The maintenance of genetic resources of orange subfamily."In : Nakatsuji, N.ed. The maintenace of genetic resources and database for life science. 68-73

Nito, N. 和 Y.Katayama：“橙子亚科遗传资源的维护”，载于：Nakatsuji, N.ed。