PHISIOLOGICAL STUDY ON PROPAGATION OF HORTICULTURAL PLANTS

园艺植物繁殖的生理学研究

• 批准号: 07045040
• 负责人: NITO Nobumasa
• 金额: $ 2.3万
• 依托单位: SAGA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07045040/
• 关键词: Vegetative propagation; Taxonomy; Adventitious bud formation; Trasformation; Hydroponic culture; Fatty acid; Agrobacterium; Genetic resources; 耐塩性; 低温ストレス; 毛状根

1. Genetic analysis of fatty acid of soybean mutants induced by X-ray radiatin revealed that the palmitic acid content was regulated by two allele.2. Somatic hybrid between cultivated onion and wild species of Allium oschinii was produced by protoplast fusion for the first time. The somatic hybrids are genetically stable and have high ability for masspropagation.3. Adventitious bud formation from shoot segment of torenia is stimulated by wounding. The secretion from wounded stem contained high molecular substances related to RNA.The identification of the substances is carrying out.4. Hairy roots from seedling of sunflower were induced by the inoculation of Agrobacterium rhizogenes MAFFO1724. The second metabolites in hairy roots produced phenol compounds tolerant to heavey metals.5. Conditions of low night humidity and high night humidity were imposed on tomato and strawberry plants. Vegetative growth of tomato plants increased with high night humidity whereas the strawberry plants did not show any incrase. The concentration of N,P,K,Ca and Mg were reduced in tomato plants under high night humidity. For strawberry plants, the high night humidity tended to increasee the concentration of majour elements.6. Rooting ability of 21 genera 48 spcies were investigated. IBA and NAA stimulated the rooting. Rooting ability were influenced by endogeous condition of plant materials.

1.对X射线诱变大豆突变体脂肪酸的遗传分析表明，棕榈酸含量受两个等位基因调控.利用原生质体融合技术首次获得了栽培洋葱与野生葱的体细胞杂种。体细胞杂种遗传稳定，大量繁殖能力强.猪蓝草茎段的不定芽发生受创伤刺激。伤茎分泌物中含有与RNA相关的高分子物质，鉴定工作正在进行中.用发根农杆菌MAFFO 1724诱导向日葵幼苗产生毛状根。毛状根次生代谢产物产生对重金属有耐性的酚类化合物.对番茄和草莓植株施加低夜间湿度和高夜间湿度的条件。番茄植株的营养生长随夜间高湿度的增加而增加，而草莓植株没有表现出任何增加。夜间高湿降低了番茄植株体内N、P、K、Ca和Mg的含量。对于草莓植株来说，夜间高湿度有增加其主要元素含量的趋势.对21属48种植物的生根能力进行了研究。IBA和NAA对生根有促进作用。生根能力受植物材料内部营养条件的影响。

项目成果

Nishikawa, K. and K. Ishimaru: "Flavonoids in root cultures of Scutellaria baicalensis." Journal or plant Physiology. 151. 633-636 (1997)

Nishikawa, K. 和 K. Ishimaru：“黄芩根培养物中的类黄酮。”

Rahman, S. M. and Y. Takagi: "Inheritance of reduced linolenic acid content in soybean oil." Ther. Appl. Genet.94. 299-302 (1997)

Rahman, S. M. 和 Y. Takagi：“大豆油中亚麻酸含量降低的遗传。”

Shigyo, M., Y.Tashiro, S.Isshiki and S.Miyazaki: "Establishment of series of alien monosomic addition lines of Japanese bunching onion (Allium fistulosum L.) with extra chromosomes from shallot (Allium cepa L.Aggregatum group)." Genes Genet.Syst.71. 363-3

Shigyo, M.、Y.Tashiro、S.Isshiki 和 S.Miyazaki：“利用来自青葱（Allium cepa L.Aggregatum 群）的额外染色体，建立了日本束洋葱（Allium fistulosum L.）的一系列外来单体附加系。

Choi, J.H., G.C.Chug and S.R.Suh: "Effect of night humidity on the vegetative growth and the mineral composition of tomato and strawberry plants." Scientia Hortic.70. 293-299 (1997)

Choi, J.H.、G.C.Chug 和 S.R.Suh：“夜间湿度对番茄和草莓植物的营养生长和矿物质成分的影响。”

Shigyo,M.,Y.Tashiro,S.Isshiki and S.Miyazaki: "Chromosomal locations of five isozyme gene loci (Lap-1,Got-1,6-Pgdh-2,Adh-1 and Gdh-1) in shallot (Allium cepa L.Aggregatum group." Japan.J.Genet.70. 399-407 (1995)

Shigyo，M.，Y.Tashiro，S.Isshiki 和 S.Miyazaki：“葱中五个同工酶基因位点（Lap-1、Got-1,6-Pgdh-2、Adh-1 和 Gdh-1）的染色体位置