Mechanisms for Inhibition of Vitellogenin synthesis by Aquatic Pollutants in the Primary Culture of Hepatocytes in Fish

水生污染物抑制鱼类肝细胞原代培养中卵黄蛋白原合成的机制

• 批准号: 10460078
• 负责人: MUGIYA Yasuo
• 金额: $ 6.59万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10460078/
• 关键词: Rainbow trout; hepatocyte culture; estrogen; vitellogenin; E2 receptor; Aluminium; Cadmium; Protein kinase C; m-RNA; A23187; 環境汚染; 細胞培養; カルシウム; mRNA

In this year, it was mainly examine if Al and Cd affect vitellogenin (VTG) synthesis at estrogen receptor (ER) and/or posttranslational levels. The results are :1. Al and Cd inhibited VTG synthesis by affecting ER levels. However, these metals had no effect on affinity between estrogen (E2) and ER, suggesting that Al and Cd inhibited the expression of ER itself by E2.2. Al inhibited protein kinase C (PKC) activity in the liver without any effects on VTG production. Therefore, Al inhibits VTG production through processes that are unrelated to PKC at a posttranslational level.3. 2,4-Dichlorophenoxyacetic acid, an environmental pollutant, induced weak VTG production in hepatocyte culture.4. An in vivo experiment showed no effect of Al on VTG synthesis in goldfish. Concentration-dependent effects of Al should be examined.These results, together with the results obtained in the last year, show that Al and Cd inhibits VTG synthesis at both ER and translational levels.

本研究主要从雌激素受体（ER）和/或翻译后水平探讨铝、镉对卵黄蛋白原（VTG）合成的影响。结果表明：1. Al和Cd通过影响ER水平抑制VTG合成。然而，这些金属对雌激素（E2）和ER之间的亲和力没有影响，这表明铝和镉抑制E2.2的ER本身的表达。铝抑制蛋白激酶C（PKC）的活性在肝脏中没有任何影响VTG的生产。因此，铝抑制VTG的生产通过过程是无关的PKC在翻译后水平。环境污染物2，4-二氯苯氧乙酸诱导肝细胞培养产生弱的VTG.体内实验表明，铝对金鱼的VTG合成没有影响。这些结果，连同去年获得的结果，表明铝和镉抑制VTG合成在ER和翻译水平。

项目成果

Yeo I.Y.: "Effects of extracellular calcium concentrations and calcium antagonists on vitellogenin induction by estradiol-17β in primary hepatocyte culture"Fisheries Science. 63(3). 443-447 (1998)

Yeo I.Y.：“细胞外钙浓度和钙拮抗剂对原代肝细胞培养物中雌二醇-17β诱导卵黄蛋白原的影响”，渔业科学 63(3) (1998)。

Hwang,U.G.: "Aluminium and cadmium inhibit vitellogenin and its mRNA induction of estradiol-17β in the primary culture of hepatocytes in the rainbow trout"General and Comparative Endocrinology. 119(1). 67-76 (2000)

Hwang, U.G.：“铝和镉抑制虹鳟肝细胞原代培养物中卵黄蛋白原及其 mRNA 诱导”，《普通与比较内分泌学》119(1) (2000)。

Hwang, W.U.: "Aluminium and cadmium inhibit vitellogenin and its mRNA induction of estradiol-17β in the primary culture of hepatocytes in the rainbow trout"General and Comparative Endocrinology. 119(1). 67-76 (2000)

Hwang, W.U.：“铝和镉抑制虹鳟肝细胞原代培养物中卵黄原蛋白及其 mRNA 诱导作用”，《普通与比较内分泌学》119(1) (2000)。

Hwang, U.G.: "Aluminium and cadmium inhibit vitellogenin and its mRNA induction of estradiol-17β in the primary culture of hepatocytes in the rainbow trout"General and Comparative Endocrinology. 119(1). 67-76 (2000)

Hwang, U.G.：“铝和镉抑制虹鳟肝细胞原代培养物中卵黄蛋白原及其 mRNA 诱导”，《普通与比较内分泌学》119(1) (2000)。

Mugiya Y.: "Inhibitory effects of aluminium on vitellogenin induction by estradiol-17 β in the primary culture of hepatocytes in the rainbow trout Oncorhynchus mykiss" General and Compartive Endocrinology. 109(1). 37-43 (1998)

Mugiya Y.：“虹鳟鱼肝细胞原代培养物中铝对雌二醇-17 β 卵黄蛋白原诱导的抑制作用”，《一般与比较内分泌学》109(1)。