VITELLOGENIN SYNTHESIS AND REGULATION BY PITUITARY HORMONES IN HEPATOCYTE CULTURES IN FISH

鱼肝细胞培养物中卵黄原蛋白的合成和垂体激素的调节

• 批准号: 05454088
• 负责人: MUGIYA Yasuo
• 金额: $ 4.42万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454088/
• 关键词: HEPATOCYTE CULTURE; VITELLOGENIN; ESTROGEN; PITUITARY HORMONE; CALCIUM; OUABAIN; EEL; RAINBOW TROUT; ランタン; 成長ホルモン; プロラクチン; 肝細胞; 培養

Vitellogenin (Vg) induction by estradiol-17 beta (E_2) was examined in primary hepatocyte cultures using rainbow trout and eels. Special attention was directed to elucidating the involvement of pituitary hormones and extracellular and intracellular Ca ions in Vg synthesis by E_2. The following results have been obtained.1. The addition of E_2 to the incubation medium actively induced Vg synthesis in rainbow trout, while the same treatment failed in Vg induction in eels. The addition of E_2 with growth hormone and/or prolactin markedly stimulated Vg synthesis in eels.2. Although the administration of E_2 resulted in Vg synthesis in sham-operated eels, no Vg synthesis occurred in hypophysectomized eels.3. When Ca concentrations in the incubation medium were changed between O and 10 mM,Vg synthesis was in a concentration-dependent way. The maximum synthesis was obtained at 5mM Ca.4. Vg synthesis was peculiarly inhibited by the addition of ouabain or La to the incubation medium. proteins other than Vg were hardly affected by these reagents.These results directly indicate the involvement of pituitary hormones, at least in eels, and calcium ions in Vg synthesis. These findings contribute to the elucidation of mechanisms of Vg synthesis in fish.

研究了雌二醇-17 β (E_2)在虹鳟和鳗鱼原代肝细胞培养中诱导卵黄原蛋白（Vg）的作用。特别注意的是阐明垂体激素和细胞外和细胞内钙离子在E_2合成Vg中的作用。得到了以下结果：1。在培养液中添加E_2对虹鳟的Vg合成有积极的诱导作用，而对鳗鱼的Vg诱导作用无效。在E_2中添加生长激素和/或催乳素可显著刺激鳗鱼体内Vg的合成。虽然给药E_2可以在假操作的鳗鱼中合成Vg，但在去垂体的鳗鱼中没有合成Vg。当培养介质中Ca浓度在0 ~ 10 mM之间变化时，Vg的合成呈浓度依赖性。在5mM Ca.4下合成量最大。在培养液中加入瓦巴因或La可特别抑制Vg的合成。除Vg以外的蛋白质几乎不受这些试剂的影响。这些结果直接表明，至少在鳗鱼中，垂体激素和钙离子参与了Vg的合成。这些发现有助于阐明鱼体内Vg合成的机制。

项目成果

Kwon H.C.and Mugiya Y.: "Involvement of Growth Hormone and Prolactin in the Induction of Vitellogenin Synthesis in Primary Hepatocyte Culture in the Eel,Anguilla japonica" General and Comparative Endocrinology. 93. 51-60 (1994)

Kwon H.C. 和 Mugiya Y.：“生长激素和催乳素参与鳗鱼原代肝细胞培养中卵黄蛋白原合成的诱导”普通和比较内分泌学。

Kwon H.C.and Mugiya Y.: "Involvement of Growth Horm one and Prolactin in the Induction of Vitellogenin Synthesis in Primary Hepatocyte Culture in the Eel,Anguilla japonica" General and Comparative Endocrinology. 93. 51-60 (1994)

Kwon H.C. 和 Mugiya Y.：“生长激素 1 和催乳素参与鳗鱼原代肝细胞培养中卵黄蛋白原合成的诱导”普通和比较内分泌学。

Kwon H.C.and Mugiya Y.: "Involvement of Growth Hormone and Prolactin in the Induction of Vitellogenin Synthesis in Primary Hepatocyte Culture in the Eel,Anguilla japonica21GC02:General and Comparative Endocrinology" 93. 51-60 (1994)

Kwon H.C.和 Mugiya Y.：“生长激素和催乳素参与鳗鱼原代肝细胞培养中卵黄蛋白原合成的诱导，Anguilla japonica21GC02：一般和比较内分泌学”93. 51-60 (1994)

Kwon H.C.et al.: "Vitellogenin Induction by Estradiol-17β in Primary Hepatocyte Culture in the Rainbow Trout,Oncorhynchus mykiss" Comparative Biochemistry and Physiology. 104B. 381-386 (1993)

Kwon H.C. 等人：“虹鳟原代肝细胞培养中雌二醇-17β 的卵黄蛋白原诱导”，比较生物化学和生理学 104B (1993)。

Kwon H.C.and Y.Mugiya: "Involvement of growth and prolactin in the induction of vitellogenin synthesis in primary hepatocyte culture in the eel, Anguilla japonica." Gen.Comp.Endocrinol.93 (1). 51-60 (1994)

Kwon H.C. 和 Y.Mugiya：“在日本鳗鲡原代肝细胞培养物中，生长和催乳素参与诱导卵黄蛋白原合成。”