Comprehensive study for control of human herpes virus infection after organ transplantation

器官移植后控制人类疱疹病毒感染的综合研究

• 批准号: 10470181
• 负责人: ASANO Yoshizo
• 金额: $ 8.32万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470181/
• 关键词: heat-labile enterotoxin; VZV; glycoprotein; mouse; liver transplantation; bone marrow transplantation; cellular immunity; 中和抗体; 変胃易熱性下痢毒素; 細胞障害性T細胞活性

1. Examination of varicella-zoster virus(VZV)glycoproteins which induce VZV-specific cellular immunity in mice Purified VZV glycoproteins were administered to BALB/c or ICR mice with heat-labile enterotoxin mutant(LT mutant)prepared from Escherichia coli as an adjuvant via nasal route. By evaluating footpad test in vivo and thimidine uptake and IL-2 production in vitro, gE : gI and gH : gL among VZV glycoproteins induced delayed type hypersensitivity to VZV more effectively than others.2. Hunan herpesvirus 6(HHV-6)and human herpesvirus 7(HHV-7)infections after liver transplantation HHV-6 infection was observed more frequently during 2 to 4 weeks after the transplantation, that correlated with etiology unknown febrile episode among severalclinical features. About 20% of the recipients had the virus genome constantly after the transplantation. The primary HHV-6 infection of the infants frequently showed unusual clinical features of exanthem subitum(febrile episode without rash)after transplantation. It was suggested that the virus was transmitted via lymphocytes in the graft.3. HHV-6 and HHV-7 infections after bone marrow transplantation(BMT)It was suggested that evaluating HHV-6 genomes in peripheral blood mononuclear cells by real-time PCR was useful for monitoring HHV-6 infection after BMT.It was suggested that HHV-6 infection might involve skin rash observed by 4 weeks after BMT.The HHV-6 infection was observed more frequently after allogeneic BMT than after autologous BMT.

1.水痘-带状疱疹病毒（VZV）糖蛋白在小鼠中诱导VZV特异性细胞免疫的研究将纯化的VZV糖蛋白与由大肠杆菌制备的不耐热肠毒素突变体（LT突变体）作为佐剂经鼻途径给予BALB/c或ICR小鼠。通过体内足垫试验和体外Thimidine摄取试验及IL-2产生试验，VZV糖蛋白中的gE：gI和gH：gL诱导VZV迟发型超敏反应的效果较好.肝移植术后人类疱疹病毒6型（HHV-6）和人类疱疹病毒7型（HHV-7）感染肝移植术后2 ~ 4周内HHV-6感染较常见，在多种临床表现中，HHV-6感染与不明原因发热有关。约20%的受者在移植后持续携带病毒基因组。婴儿原发性HHV-6感染在移植后常表现出不寻常的皮下皮疹（无皮疹的发热发作）的临床特征。提示病毒通过移植物内淋巴细胞传播.骨髓移植后HHV-6和HHV-7感染结果提示，实时荧光定量PCR检测外周血单个核细胞HHV-6基因组有助于监测骨髓移植后HHV-6感染情况，提示HHV-6感染可累及骨髓移植后4周的皮疹，异基因骨髓移植后HHV-6感染率高于自体骨髓移植。

项目成果

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