Comprehensive study for control of human herpes virus infection after organ transplantation

1.水痘-带状疱疹病毒(VZV)诱导小鼠细胞免疫的糖蛋白检测纯化的VZV糖蛋白以大肠杆菌不耐热肠毒素突变体(LT突变体)为佐剂经鼻腔给药给BALB/c或ICR小鼠。通过体内足垫试验和体外甲硫咪胺摄取及IL-2产生情况评价，VZV糖蛋白中Ge：Gi和Gh：Gl诱导VZV迟发型超敏反应的效果最好。肝移植后湖南疱疹病毒6型(HHV-6)和人类疱疹病毒7型(HHV-7)感染以术后2~4周最多见，与病因不明发热有关。约20%的受者在移植后持续携带病毒基因组。婴儿HHV-6的原发感染在移植后常常表现出罕见的皮疹(无皮疹的发热发作)的临床特征。推测该病毒是通过移植物中的淋巴细胞传播的。骨髓移植后HHV-6和HHV-7感染提示，用实时定量聚合酶链式反应检测外周血单核细胞中HHV-6基因组可用于监测骨髓移植后HHV-6感染。提示HHV-6感染可能与骨髓移植后4周内观察到的皮疹有关，异基因骨髓移植后HHV-6感染的发生率高于自体骨髓移植后。

1. Examination of varicella-zoster virus(VZV)glycoproteins which induce VZV-specific cellular immunity in mice Purified VZV glycoproteins were administered to BALB/c or ICR mice with heat-labile enterotoxin mutant(LT mutant)prepared from Escherichia coli as an adjuvant via nasal route. By evaluating footpad test in vivo and thimidine uptake and IL-2 production in vitro, gE : gI and gH : gL among VZV glycoproteins induced delayed type hypersensitivity to VZV more effectively than others.2. Hunan herpesvirus 6(HHV-6)and human herpesvirus 7(HHV-7)infections after liver transplantation HHV-6 infection was observed more frequently during 2 to 4 weeks after the transplantation, that correlated with etiology unknown febrile episode among severalclinical features. About 20% of the recipients had the virus genome constantly after the transplantation. The primary HHV-6 infection of the infants frequently showed unusual clinical features of exanthem subitum(febrile episode without rash)after transplantation. It was suggested that the virus was transmitted via lymphocytes in the graft.3. HHV-6 and HHV-7 infections after bone marrow transplantation(BMT)It was suggested that evaluating HHV-6 genomes in peripheral blood mononuclear cells by real-time PCR was useful for monitoring HHV-6 infection after BMT.It was suggested that HHV-6 infection might involve skin rash observed by 4 weeks after BMT.The HHV-6 infection was observed more frequently after allogeneic BMT than after autologous BMT.