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Study on the associated regulation of energy and metabolism of glucose and lipids

能量与糖脂代谢相关调控研究

基本信息

批准号：
10470235
负责人：
INOUE Gen
金额：
$ 8.32万
依托单位：
Research Institute for Production Development
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

1.Using leptin-overexpressing transgenic mice and KKA^γ mice, we examined the effects of leptin on the development of obesity and diabetes. Leptin overexpressing KKA^γ mice became obese as KKA-γ mice did, while the development of diabetes in leptin overexpressing KKA^γ mice was significantly delayed compared to KKA^γ mice. The result suggests that leptin may be useful for the prevention of diabetes.2.We found that insulin stimulated IRS1-associated PI3 kinase activity was increased in liver and skeletal muscles of leptin transgenic mice and themice intra cranially injectedwithieptin. However, could not find changes in Akt activity or the expression of constituents of intracellular insulin signal in liver and skeletal muscles of leptin transgenic mice. The results imply the divergent effects of leptin on insulin signaling system.3.We already developed transgenic mice expressing UCP3 in skeletal muscles, and are going to explore the roles of energy expenditure on the regulation of glucose and lipid metabolism.Ninna-ji Temple was founded in 888 AD. by the Emperor Uda at the request of his father, the former Emperor Kk, in the northwestern suburbs of the Capital-City Heian. Ninna-ji Temple hierarchically occupied the highest position among the, nonzeki temples whose abbots were members of the Impenal Family until the end of the Edo era, therefore the research of the documents which have been preserved at Ninna-ji Temple for centuries (the Ninna-ji Documents in general) contributes not only to the historical study of the temple, but also to the study of Japanese history.

1.利用瘦素过表达转基因小鼠和KKA^γ小鼠，我们研究了瘦素对肥胖和糖尿病发展的影响。瘦素过表达的KKA^γ小鼠与KKA-γ小鼠一样肥胖，而瘦素过表达的KKA^γ小鼠与KKA^γ小鼠相比，糖尿病的发展明显延迟。结果表明，瘦素可能有助于预防糖尿病。我们发现胰岛素刺激的irs1相关PI3激酶活性在瘦素转基因小鼠和脑内注射瘦素小鼠的肝脏和骨骼肌中增加。然而，在瘦素转基因小鼠的肝脏和骨骼肌中，Akt活性和细胞内胰岛素信号成分的表达未见变化。结果提示瘦素对胰岛素信号系统的影响存在差异。我们已经在骨骼肌中培育了表达UCP3的转基因小鼠，并将探索能量消耗对糖脂代谢的调节作用。宁纳寺建于公元888年。这是由乌田天皇应其父亲，即前皇上的要求，在首都平安城的西北郊建造的。直到江户时代末期，仁寺的住持都是亡者家族的成员，因此，对仁寺保存了几个世纪的文献（一般的仁寺文献）的研究不仅有助于对寺庙的历史研究，也有助于对日本历史的研究。