Clinical significance of polymorphism in cGMP-related genes on blood pressure control in humans.

cGMP相关基因多态性对人类血压控制的临床意义。

• 批准号: 13204043
• 负责人: INOUE Gen
• 金额: $ 21.12万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13204043/
• 关键词: Hypertension; genome; natriuretic peptide; GC-A; allele frequency; 遺伝子多型; 高血圧症; CT繰り返し配列; ノックアウトマウス; 心室; 心肥大; 内皮; eNOS; 遺伝子; 本能性高血圧症; GC-A遺伝子; 転写活性; ANP; cGMP; SNP

The purpose of this study was to evaluate the significance of the effect of cGMP-related gene on controlling blood pressure in humans. After the informed consents, blood sample was drawn from normotensive (n=180, mean blood pressure 124/74 mmHg) and hypertensive (n=189, mean blood pressure 162/93 mmHg). Extracted genome was subjected to PCR direct sequence method to detect polymorphism in the sequence of guanylyl cyclase-A gene, which is one of the two major types of receptors for natriuretic peptides. As a result, we had detected 5 polymorphisms through the whole span of 22 exsons and 21 introns which length is 16kb. In this study, we investigated one of those polymorphisms, CT repeats in-300 by of 5' franking lesion of this gene. We detected the existence of (CT)6 repeats in addition to already reported (CT) 10, 11, 12 repeats. Moreover, the allele frequency of (CT)6 seems to be significantly different between normotensive and hypertensive. On the other hand, there seems to be no significant difference in the allele frequency of (CT) 10, 11, 12 repeats. We are now confirming our results and on the discussion about its significance on blood pressure control in humans

本研究的目的是评价cGMP相关基因在人类血压控制中的意义。在知情同意后，从血压正常（n=180，平均血压124/74 mmHg）和高血压（n=189，平均血压162/93 mmHg）中抽取血液样本。提取基因组DNA，采用PCR直接测序法检测尿钠肽两种主要受体之一鸟苷酸环化酶A基因序列的多态性。结果发现，在22个外显子和21个内含子的16 kb的全长范围内，共检测到5个多态性位点。在本研究中，我们研究了这些多态性之一，CT重复在-300由该基因的5'端的连锁病变。我们检测到除了已经报道的（CT）10，11，12个重复之外，还存在（CT）6个重复。此外，（CT）6的等位基因频率似乎在正常血压和高血压之间存在显著差异。另一方面，（CT）10、11、12重复的等位基因频率似乎没有显著差异。我们现在正在确认我们的结果，并讨论其对人类血压控制的意义

项目成果

Hypertrophic responses to cardiotrophin-I are not mediated by STAT3, but via a MEK5-ERK5 pathway in cultured cardiomyocytes.

对心肌营养蛋白-I 的肥大反应不是由 STAT3 介导的，而是通过培养的心肌细胞中的 MEK5-ERK5 通路介导的。

• 发表时间: 2005
• 期刊: J Mol Cell Cardiol 38
• 作者: Takahashi N et al.

Plasma level of B-type natriuretic peptide as a prongostic marker after acute myocardial infarction - A long-term follow up analysis.

B 型利尿钠肽的血浆水平作为急性心肌梗死后的预后标志物 - 长期随访分析。

• 发表时间: 2004
• 期刊: Circulation 110
• 作者: Asano;T.;藤田禎三;Suzuki S et al.
• 通讯作者: Suzuki S et al.

T.Miyawaki, et al.: "Clinical implications of leptin and its potential humoral regulators in long-term low-calorie diet therapy for obese humans"Eur.J.Clin.Nutr.. 56(7). 593-600 (2002)

T.Miyawaki 等人：“瘦素及其潜在体液调节剂在肥胖人群长期低热量饮食治疗中的临床意义”Eur.J.Clin.Nutr.. 56(7)。

Role of natriuretic peptide recepter guanylyl cyclase-A in myocardial infarction evaluated using genetically engineered mice

使用基因工程小鼠评估利尿钠肽受体鸟苷酸环化酶-A 在心肌梗死中的作用

• 发表时间: 2005
• 期刊: Hypertension 46
• 作者: Nakanishi;M et al.
• 通讯作者: M et al.

A.Yasoda, K.Nakao et al.: "Overexpression of CNP in chondrocytes rescues achondroplasia through a MAPK-dependent pathway."Nat Med.. 10(1). 80-86 (2004)

A.Yasoda、K.Nakao 等人：“软骨细胞中 CNP 的过度表达通过 MAPK 依赖性途径挽救软骨发育不全。”Nat Med.. 10(1)。