Tumor oxygenation to in crease sensitivity to anti-cancerous drug by using artificial oxygen carrier

癌细胞通常对抗癌药具有抗药性。为了研究人工氧载体(AOCs)是否增加肿瘤细胞对抗癌药物的敏感性，设计了以下实验：将吉田肉瘤细胞(LY-80)移植到大鼠背部肌肉中，用微球法对肿瘤内的血流进行了定量分析，并用氧斑法测定了肿瘤的组织氧分压(PtO2)。使用的AOCs是血红蛋白囊泡(HBVs)、白蛋白-血红素(AH)和全氟碳化合物(PFCs)。他们是被静脉注射的。对照组注射乳酸林格液和5%白蛋白溶液。在AOCs中，只有AH使PtO_2增加到基础值的130%。另一组PtO_2未见增加，可能是清除了一氧化氮，导致微血管收缩导致血流减少。要解决这一机制，还需要进一步的实验来研究肿瘤的微血管循环。此外，动脉注射AOCs和抗癌药物也必须进行评估。

Cancer cells are often resistant to anti-cancerous agents. One of the reasons of this phenomenon is cancer cells are hypoxic.To study if artificial oxygen carriers (AOCs) increases sensitivity of tumor cells to anti-cancerous agents, following experiments were designed.Yoshida sarcoma cells (LY-80) were inplanted to the back muscle of rats.Quantative analysis of blood flow in the tumors was performed by microsphere method.Using Oxyspot method, tissue oxygen pressure of the tumors (PtO_2) was evaluated. The AOCs used were hemoglobin vesicles (HbV), albumin-heme (AH) and perfluorocarbons (PFCs). They were intravenously injected. As control, Ractate Ringer solution and 5% albumin solution were injected. Among AOCs, only AH increased PtO_2 upto 130% of the basal value. In another groups PtO_2 was not increased, probably become they scavenged nitric oxide to cause microvascular contraction to decrease blood flow.To resolve this mechanism further experiments are needed to study microvascular circulation in the tumors. Also arterial infusion of the AOCs and anti-cancerous drugs must be evaluated.