Study of expression of NMDA receptors molecular-biologically and the pathophysiological roles in the lung

NMDA 受体表达的分子生物学研究及其在肺中的病理生理作用

• 批准号: 10470321
• 负责人: KUDOH Ichidai
• 金额: $ 5.38万
• 依托单位: YOKOHAMA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470321/
• 关键词: LUNG; NMDA; RECEPTOR; NR2C; NR2D; RT-PCR; ノーザンブロット法; 肺障害

The expression of mRNAs of the N-methyl-D-aspartate (NMDA) receptors in the trachea and lung of a rat was investigated molecular-biologically. The NMDA receptor subunit NR2C was detected in the lung, and NR2D in the trachea and lung by reverse transcriptase-polymerase chain reaction (PCR). The individual PCR products were cloned and confirmed by sequencing. Messenger RNAs purified from total RNA were subjected to Northern blot analyses. The NR2C mRNAs was detected weekly and NR2D mRNAs was detected as a minor band in the adult and neonate lungs by Northern blot analysis. Messenger RNA of NR1 was not detected in these organs.The roles of NMDA receptors in the lung were investigated using pharmacological and physiological methods. But any significant changes were detected by those methods.Messenger RNAs of NMDA receptors exist in the lung, but their pathophysiological roles were not detected in this study.

对大鼠气管和肺中 N-甲基-D-天冬氨酸 (NMDA) 受体 mRNA 的表达进行了分子生物学研究。通过逆转录聚合酶链反应（PCR）在肺中检测到 NMDA 受体亚基 NR2C，在气管和肺中检测到 NR2D。克隆各个 PCR 产物并通过测序进行确认。从总 RNA 中纯化的信使 RNA 进行 Northern 印迹分析。每周检测一次 NR2C mRNA，并通过 Northern 印迹分析检测成人和新生儿肺中的 NR2D mRNA 作为次要条带。在这些器官中未检测到NR1的信使RNA。采用药理学和生理学方法研究了NMDA受体在肺中的作用。但这些方法均未检测到任何显着变化。肺中存在NMDA受体的信使RNA，但本研究未检测到其病理生理作用。

项目成果

I Kudoh, , H Miyazaki, H Yamada, J Fujimoto, M Ohara, T Tazawa, J Fukushima, F Okumura: "Pretreatment of acid-induced lung injury with specific neutrophil inhibitor ONO-5046 did not exasperate the subsequent lung infection by Pseudomonas aeruginosa."Masui

I Kudoh、H Miyazaki、H Yamada、J Fujimoto、M Ohara、T Tazawa、J Fukushima、F Okumura：“用特异性中性粒细胞抑制剂 ONO-5046 预处理酸诱导的肺损伤不会加剧随后的铜绿假单胞菌肺部感染

H Sato, Si Hattori, S Kawamoto, IKudoh A Hayashi, I Yamamoto, M Yoshinari, M Minami, H Kanno.: "In vivo gene gun-mediated DNA delivery into rodent brain tissue."Biochem Bioph Res Co. 270. 163-170 (2000)

H Sato、Si Hattori、S Kawamoto、IKudoh A Hayashi、I Yamamoto、M Yoshinari、M Minami、H Kanno.：“体内基因枪介导的 DNA 递送到啮齿动物脑组织中。”Biochem Bioph Res Co. 270. 163-

K Xin: "Evidence of HIV 1 glycoprotein 120 binding to recombinant N-methy-D-aspartate receptor subunits expressed in a baculovirus system."AIDS research and human retroviruses. 16. 1461-1467 (1999)

K Xin：“HIV 1 糖蛋白 120 与杆状病毒系统中表达的重组 N-甲基-D-天冬氨酸受体亚基结合的证据。”艾滋病研究和人类逆转录病毒。

S Kawamoto, S Hattori, S Uchino, M Mishina, K Okuda: "Expression of NMDA receptor channel subunit proteins using baculovirus and herpes virus vectors."Method in Molecular Biology. 128. 19-32 (1999)

S Kawamoto、S Hattori、S Uchino、M Mishina、K Okuda：“使用杆状病毒和疱疹病毒载体表达 NMDA 受体通道亚基蛋白。”分子生物学方法。

Hiroshi Yamada, Ichidai Kudoh, Hiroshi Miyazaki, Tatsuaki Kikuchi, Junich Fujimoto.: "Acid aspiration enhances the inflammatory response to subsequent lipopolysaccharide challenge in rats."Am J Respir Crit Care Med Am.J.Respir. 162. 1366-1371 (2000)

Hiroshi Yamada、Ichidai Kudoh、Hiroshi Miyazaki、Tatsuaki Kikuchi、Junich Fujimoto.：“酸吸入增强了大鼠对随后脂多糖挑战的炎症反应。”Am J Respir Crit Care Med Am.J.Respir。