T-cell targeting treatment strategy of infective or allergic inflammation in nasopharyrix-an analysis of human T -lymphocytes recruting to nasopharyngeal mucosal linings-

我们利用分子生物学技术和TCR转基因小鼠、IL-5转基因小鼠、NOD-SCID小鼠等实验动物，从免疫学的角度探讨了人和啮齿动物鼻咽粘膜免疫诱导和维持的确切机制。在动物实验中，我们已经阐明了T细胞和B细胞对于诱导和维持抗原特异性的粘膜抗体应答是必不可少的，这些T细胞从细胞因子的角度进行修饰可以控制鼻咽粘膜的过敏性或感染性炎症。另一方面，我们对人类样本的定量RT-PCR分析显示，鼻变态反应性鼻炎患者和感染性鼻炎患者鼻黏膜中的T细胞细胞因子谱存在一定的差异，人扁桃体淋巴细胞对NOD-SCID小鼠的转移研究表明，根据持续的抗原刺激，人扁桃体淋巴细胞已被承诺为抗体产生B细胞和记忆T细胞。综上所述，这些结果可能会鼓励我们进行更广泛的研究，以追求其在鼻咽炎性疾病治疗中的新策略和临床应用。

We have immunologically examined the precise mechanism of induction and maintenance of mucosal immunity in nasopharynx of human-beings and rodents as well, by employing molecular biology techniques and experimental animals such as TCR-transgenic mice, IL-5 transgenic mice, and NOD-scid mice. In animal experiments, we have elucidated that T cells recruting to the nasopharyngeal mucosa as well as B cells are essential for an induction and maintenance of antigen-specific mucosal antibody response and that these T-cell modification in view of cytokine profile may control the scenario of allergic or infective inflammation in nasopharyngeal mucosa. On the other hand, our quantitative RT-PCR analysis with human samples revealed that T-cell cytokine profile in nasal mucosae is different somehow between patients with nasal allergy and those with infective rhinitis and a transfer study of human tonsillar lymphocytes to NOD-scid mice demonstrated that human tonsillar lymphocytes have been committed to be antibody-prodecing B cells and memory T cells according to the continuous antigenic stimuli. These results taken together might encourage our extensive research for pursuiting the new strategy and clinical application of it to the treatment of inflammatory diseases in nasopharynx.