Studies on the inner ear immune

以小鼠、大鼠和毛丝鼠为实验动物，探讨内耳免疫应答的机制。（1）在无菌、无特定病原体和常规三种不同条件下饲养的小鼠中，检测了内淋巴囊（ELsac）中免疫活性细胞的分布，该免疫活性细胞被证明是内耳局部免疫应答所必需的。(2)In在另一个实验中，在全身性预致敏后进行外淋巴抗原攻击的SPF大鼠中检查淋巴细胞亚群的募集。（3）将流感嗜血杆菌内毒素注入灰鼠中耳，观察中耳内毒素对内耳的影响。这些结果使我们得出以下结论。ELsac最初并不具备免疫活性细胞并产生免疫应答，但一旦它被内耳抗原刺激物激活，ELsac就可以成为内耳局部免疫应答的活性部位。中耳中存在的内毒素可以渗透圆窗膜，并且可以引起内耳炎症反应和内淋巴积水，即使目前的数据不能直接外推到人类状况。

The mechanism of inner ear immune response was investigated, employing mice, rats, and chinchillas as experimental animals.(1)The distribution of immunocompetent cells in endolymphatic sac (ELsac), Which is proven to be essential for local immune response of the inner ear, was examined in mice bred in three different condition ; germ-free, specific-pathogen free, and conventional.(2)In another experiment, the recruitment of lymphocyte subsets was examined in SPF rats undergoing a perilymphatic antigen challenge after systemic presensitization.(3)The effect of middle ear endotoxin on the inner ear was examined in chinchillas underwent an instillation of endotoxin derived from H.influenzae to the middle ear. The results taken lead us to the following conclusion. The ELsac is not originally equipped to possess immunocompetent cells and mount an immune response, but once it has been activated with inner ear antigenic stimuli, the ELsac can be the active site of a local immune response of the inner ear. Endotoxin present in the middle ear can permeate the round window membrane and can cause the inner ear inflammatory response and endolymphatic hydrops, even though the present data can not be directly extrapolated into human conditions.