Morphologic and Electrophysiologic Study of Retinal Pigment Epithelial Cell Transplantation in Age-Related Macular Degeneration

年龄相关性黄斑变性视网膜色素上皮细胞移植的形态学和电生理学研究

• 批准号: 10470367
• 负责人: TAKEUCHI Shinobu
• 金额: $ 4.74万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470367/
• 关键词: Age-related macular degeneration; Retinal pigment epithelial transplantation; 網膜移植; 網膜細胞移植; 自家周辺部移植

1) The retinal pigment epithelium cells together with the choroid were obtained from eyeballs through the scleral opening successfully accompanied with minor bleedings. Subsequent retinal detachment was not observed by the fundoscopic examination.2) Resected specimens were enzymaticaly treated with collagenase in order to isolate the pigment epithelial cells. Marked intra- and inter-experimental differences were observed in isolating the cells, and the repeatable method for isolation has not been established yet.3) The retinal pigment epithelial cells derived from peripheral areas were transplanted in the subretinal space in normal rabbits in cell suspension or in a form of a single sheet transvitreally. No serious complication such as immune rejection was observed postoperatively, confirming the safety of the transplantation surgery.4) The TGF-beta neutralizing antibody successfully suppresses the development of experimental proliferative vitreoretinopathy in rabbit eyes.5) Electroretinograms were examined in patients with various retinal diseases to study retinal function, in order to compare with those in experimental animals.6) An LED built-in electrode was developed and tested in human eyes.

1)经巩膜开口处成功获得视网膜色素上皮细胞和脉络膜，并伴有少量出血。2)切除标本用胶原酶处理，分离色素上皮细胞。细胞的分离存在明显的实验内和实验间差异，目前还没有建立可重复的分离方法。3)将来源于周边区域的视网膜色素上皮细胞以细胞悬液或单层玻璃体移植到正常兔的视网膜下间隙。术后未观察到免疫排斥等严重并发症，证实了移植手术的安全性。4)转化生长因子-β中和抗体成功地抑制了实验性增殖性玻璃体视网膜病变的发展。5)对各种视网膜疾病患者进行了视网膜电图检查，以研究视网膜功能，并与实验动物的视网膜功能进行了比较。6)研制了LED内置电极，并在人眼进行了测试。

项目成果

Mitamura Y: "Macrophage migration inhibitory factor level in the vitreous"American Journal of Ophthalmology. 129. 678-680 (2000)

Mitamura Y：“玻璃体内巨噬细胞迁移抑制因子水平”美国眼科杂志。

Yamamoto S: "Cone electroretinogram amplitude growth with light adaptation in patients with retinitis pigmentosa."Acta Ophthalmologica Scandinavia. 78. 403-406 (2000)

Yamamoto S：“色素性视网膜炎患者的视锥细胞视网膜电图振幅随光适应的增长。”斯堪的纳维亚眼科学报。

Yamamoto S,et al: "Electroretinogram and visual evoked potentials eliuted by spectral stimuli in a patient with enhanced S-coue syndreme"Jpn J Ophthalmol. 43. 433-437 (1999)

Yamamoto S 等人：“患有增强型 S-coue 综合征的患者通过光谱刺激洗脱的视网膜电图和视觉诱发电位”Jpn J Ophthalmol。

Yamamoto S: "Cone electroretinogram amplitude growth with light adaptation in patients with retinitis pigmentosa"Acta Ophthalmologica Scandinavica. 78. 403-406 (2000)

Yamamoto S：“色素性视网膜炎患者的锥体视网膜电图振幅随光适应的增长”《斯堪的纳维亚眼科学报》。

Mitamura Y: "Macrophage migration inhibitory factor levels in the vitreous of patients with proliferative vitreoretinopathy"American Journal of Ophthalmology. 128. 793-765 (1999)

Mitamura Y：“增殖性玻璃体视网膜病变患者玻璃体中巨噬细胞迁移抑制因子的水平”美国眼科杂志。