The suppression of allograft rejection in retinal pigment epithelial transplantation with ACAID.

ACAID 抑制视网膜色素上皮移植中的同种异体移植排斥反应。

• 批准号: 09671799
• 负责人: TANO Tasuo
• 金额: $ 1.66万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671799/
• 关键词: ACAID; allograft rejection; retinal pigment epithelium; TGF-beta; 前房関連性免疫偏位; TGF-β

A variety of antigens inoculated into the anterior chamber (AC) of the eye can elicit systemic suppression of T cell-mediated delayed-type hypersensitivity (DTH). This phenomenon is called anterior chamber-associated immune deviation (ACAID). In this research project, we first examined whether corneal allograft rejection can be suppressed by the induction of ACAID. Splenocytes collected from B10.D2 mouse were injected into the AC of BALB/c mouse. Seven days later, BALB/c mice were immunized subcutaneously by B10.D2 splenocytes. Seven days later, DTH was evaluated by ear swelling response. The eyes of BALB/c mice were grafted with B10.D2 corneal lenticles and alloepithelial rejection was evaluated using the scoring system. The results showed that AC inoculation of splenocytes induced antigen-specific of DHT response as well as supression of epithelial rejection.In contrast to AC inoculusion of splenocytes, AC inoculusion of retinal pigment epithelium (RPE) collected from Lewis rat nor B10.D2 mouse did not induce ACAID. Our results suggested that RPE could not be antigen presenting cell in retina.

前房(AC)接种多种抗原可诱导全身抑制T细胞介导的迟发型超敏反应(DTH)。这种现象被称为前房相关性免疫偏离(ACAID)。在本研究项目中，我们首先研究了ACAID的诱导能否抑制同种异体角膜移植排斥反应。将取自B10.D2小鼠的脾细胞注入BALB/c小鼠的AC。7天后，用B10.D2脾细胞皮下免疫BALB/c小鼠。7天后，采用耳廓肿胀反应评价迟发型超敏反应。将BALB/c小鼠的眼内植入B10.D2角膜晶状体，用评分系统评价同种异体上皮排斥反应。结果表明，AC接种脾细胞可诱导抗原特异性的DHT反应并抑制上皮细胞排斥反应，而Lewis大鼠和B10.D2小鼠的视网膜色素上皮(RPE)AC接种与AC接种相比，不能诱导ACAID。结果提示，RPE不能作为视网膜的抗原提呈细胞。

项目成果

Miyazaki D: "T-cell mediated immune responses in alloepithelial rejection after murine keratoepithelioplasty."Invest Ophthalmol Vis Sci. 40. 2590-2597 (1999)

Miyazaki D：“鼠角膜上皮成形术后同种异体上皮排斥反应中 T 细胞介导的免疫反应。”Invest Ophasemol Vis Sci。

Miyazaki D: "Neutrophil chemotaxis induced by corneal epithelial cells after herpes simplex virus type 1 infection."Curr. Eye Res.. 17. 687-693 (1998)

Miyazaki D：“1 型单纯疱疹病毒感染后角膜上皮细胞诱导的中性粒细胞趋化性。”Curr。

Yao Y-F: "The antigen-bearing eye and the spleen are indispensable in maintaining anterior chamber-associated immune deviation"Invest Ophthalmol Vis Sci. 38. 534-539 (1997)

姚永峰：“携带抗原的眼和脾对于维持前房相关的免疫偏差是不可缺少的”Invest Ophthalmol Vis Sci。

Yao Y-F: "Correlation of anterior chamber-associated immune deviation with suppression of corneal epithelial rejection in mice."Invest. Ophthal. Vis. Sci. 38. 292-300 (1997)

姚永峰：“小鼠前房相关免疫偏差与抑制角膜上皮排斥反应的相关性。”Invest。

Miyazaki D: "T-cell mediated immune responses in alloepithelial rejection afetr murine keratoepithelioplasty"Invest Ophthalmol Vis Sci. 40. 2590-2597 (1999)

Miyazaki D：“鼠角膜上皮成形术后同种上皮排斥中 T 细胞介导的免疫反应”Invest Ophasemol Vis Sci。