The suppression of allograft rejection in retinal pigment epithelial transplantation with ACAID.

将多种抗原接种到眼前房（AC）中可以引起T细胞介导的迟发型超敏反应（DTH）的全身抑制。这种现象被称为前房相关免疫偏离（ACAID）。在本研究项目中，我们首先研究了是否可以通过诱导ACAID来抑制角膜移植排斥反应。将从B10.D2小鼠收集的脾细胞注射到BALB/c小鼠的AC中。7天后，用B10.D2脾细胞皮下免疫BALB/c小鼠。7天后，通过耳肿胀反应评价DTH。将B10、D2角膜移植片移植于BALB/c小鼠眼内，采用角膜移植排斥反应评分系统评价同种异体角膜移植排斥反应。结果表明，AC接种脾细胞可诱导抗原特异性的DHT反应，并抑制上皮细胞的排斥反应，而AC接种刘易斯大鼠和B10.D2小鼠的视网膜色素上皮细胞（RPE）则不能诱导ACAID。结果提示，RPE细胞不是视网膜的抗原提呈细胞。

A variety of antigens inoculated into the anterior chamber (AC) of the eye can elicit systemic suppression of T cell-mediated delayed-type hypersensitivity (DTH). This phenomenon is called anterior chamber-associated immune deviation (ACAID). In this research project, we first examined whether corneal allograft rejection can be suppressed by the induction of ACAID. Splenocytes collected from B10.D2 mouse were injected into the AC of BALB/c mouse. Seven days later, BALB/c mice were immunized subcutaneously by B10.D2 splenocytes. Seven days later, DTH was evaluated by ear swelling response. The eyes of BALB/c mice were grafted with B10.D2 corneal lenticles and alloepithelial rejection was evaluated using the scoring system. The results showed that AC inoculation of splenocytes induced antigen-specific of DHT response as well as supression of epithelial rejection.In contrast to AC inoculusion of splenocytes, AC inoculusion of retinal pigment epithelium (RPE) collected from Lewis rat nor B10.D2 mouse did not induce ACAID. Our results suggested that RPE could not be antigen presenting cell in retina.