Characterization of entomopathogenic viruses for their use as pest control agents

如果病毒已经被鉴定，那么昆虫病原病毒作为害虫控制剂的使用更有可能成功。对从重要农业害虫中分离到的昆虫病原病毒进行了鉴定。所得结果总结如下.测定了甜菜夜蛾核型多角体病毒（Spodoptera exigua nucleopolyhedrovirus，NPV）、斜纹夜蛾核型多角体病毒（Spodoptera litura NPV）、粘虫分离核型多角体病毒（Mythimna separate NPV）、本麦夜蛾核型多角体病毒（Spodoptera honmai NPV）、本麦夜蛾颗粒体病毒（A.honmai granulovirus，GV）、大瘤人夜蛾颗粒体病毒（Homona magnanima GV）、海岛群岛群岛虫（Archips insulanus GV）、本麦夜蛾昆虫痘病毒（A.honmai entomopoxvirus，EPV）、粘虫分离EPV对寄主昆虫幼虫的致死剂量和存活时间.我们通过噬斑纯化法克隆了斜纹夜蛾核型多角体病毒和甜菜夜蛾核型多角体病毒。我们还测定了每个克隆在不同昆虫细胞系中的出芽病毒和多角体蛋白的产生以及DNA复制。对小菜蛾GV和玉米斑螟GV基因组DNA进行测序，并将预测的开放阅读框与其他杆状病毒基因进行比较.我们建立了从日本昆虫中分离的昆虫病原病毒数据库

The use of entomopathogenic viruses as pest control agents is much more likely to be successful if the virus has been characterized. We characterized some of entomopathogenic viruses that had been isolated from important agriculture pests. The summary of obtained results is as follow.1. We determined the lethal doses and survival times of Spodoptera exigua nucleopolyhedrovirus(NPV), Spodoptera litura NPV, Mythimna separate NPV, Adoxophyes honmai NPV, A.honmai granulovirus(GV), Homona magnanima GV, Archips insulanus GV, A.honmai entomopoxvirus(EPV), M.separate EPV for host insect larvae.2. We cloned S.litura NPV and S.exigua NPV by plaque purification. We also determined production of the budded viruses and polyhedrin and DNA replication of each obtained clone in various insect cell lines.3. The genoma DNA from Plutella xylostella GV and Xestia c-nirum GV was sequenced and the predicted open reading frames were compared genes from other baculoviruses.4. We constructed the database of entomopathogenic viruses isolated from Japanese insects