DNA sequence of a protein lethal to an endoparasitoid, Cotesia kariyai, encoded in genome of entomopoxvirus amd its spectrum on another endoparasitoid, Meteorus pulchricornis

卡氏盘绒茧蜂（Cotesia kariyai）是一种寄生昆虫痘病毒（MyseEPV）感染的粘虫（Mythimna separata）幼虫的寄生蜂。先前的研究表明，一种对卡氏盘绒茧蜂致死的毒性因子存在于感染MyseEPV的粘虫幼虫的无病毒血浆（VFP）中。本研究从MyseEPV感染的幼虫中分离到一个分子量为28-kDa的多肽，命名为PLCK（Protein Lethal to C.kariyai）。pick的ORF为1265 bp，在Xestia c-nigram（鳞翅目：夜蛾科）颗粒体病毒和Amsacta moori EPV基因组中发现了其同源序列，但PLCK是否对卡氏夜蛾以外的寄生蜂有影响尚不清楚。在这项研究中，我们研究了PLCK的影响，流星pulchrcornis（Hyplutera：茧蜂科）。与C.kaniyai一样，M.pulchricornis也是一个茧蜂末端 ...更多信息 寄生虫样;然而，它是一个多面手，不仅寄生粘虫幼虫，而C.kariyai是一个专家，因为只寄生粘虫幼虫。我们观察了这两种寄生蜂暴露于PLCK后的幼虫发育和存活，在体内和体外。感染MyseEPV的粘虫幼虫中未出现卡氏隐翅虫幼虫。而感染MyseEPV或注射VFP的粘虫幼虫的羽化率、化蛹率和成虫羽化率与未感染的粘虫幼虫和注射健康昆虫血淋巴的粘虫幼虫的羽化率和成虫羽化率差异不显著。同样，丽角小蠊幼虫在含有PLCK的IPL-41培养基中存活7天，而卡里瓦尔小蠊在同一培养基中在2天内死亡，表现出角质层从体组织上脱落的特征性症状。我们还检测了PLCK在寄生蜂幼虫中的免疫组织化学定位，以确定PLCK是否附着在C. kariyai，而不是在M. pulchricornis。总之，PLCK对美丽角小蠊的生长发育和存活没有影响，但对嘉利谷小蠊有致死作用。少

When the gregarious endoparasitoid Cotesia kariyai (Hymenoptera : Braconidae) parasitizes Mythimna separata(Lepidoptera : Noctuidae) larvae that are infected with an entomopoxvirus (MyseEPV), the embryos and larvae of the parasitoid die inside the host before emergence. Previous studies showed that a toxic factor, lethal to Cotesia kariyai, was present in virion-free plasma (VFP) from M.separata larvae infected with MyseEPV. A polypeptide of 28-kDa was purified and designated as Protein Lethal to C.kariyai (PLCK).In this study, we isolated PLCK from MyseEPV infected larvae and determined DNA sequence of a gene encoding PLCK. ORF of pick is 1265bp, and its homologues were found in genomes of Xestia c-nigram (Lepidotera : Noctuidae) granulovirus and Amsacta moori EPV.It has not been known whether PLCK affects parasitoids other than C.kariyai. In this study we examined the effect of PLCK on Meteorus pulchrcornis (Hymenoptera : Braconidae). Like C.kaniyai, M.pulchricornis is a braconid end … More oparasitoid ; however, it is a generalist, parasitizing not only M.separata larvae, whereas C.kariyai is a specialist as only parasitizes M.separata larvae. We observed larval development and survival of these two parasitoids after exposure to PLCK, both in vivo and in vitro. No C.kariyai larvae emerged from M.separata larvae infected with MyseEPV. However, percentages of emergence, pupation and adult eclosion of M.pulchricornis larvae developed in MyseEPV-infected or VFP-injected M.separata larvae were not significantly different from those in non-infected M.separata larvae or that injected with hemolymph from healthy insects. Similarly, M.pulchricornis larvae survived for seven days in IPL-41 medium containing PLCK, but C.karival died within two days in the same medium, showing pathognomonic symptom as the cuticle detachment from the somatic tissues. We also examined immunohistological localization of PLCK within parasitoid larvae developing in medium containing PLCK, to determine whether PLCK attaches to particular tissues in C. kariyai but not in M.pulchricornis. In summaly, PLCK has no effect on the development and survival of M.pulchricornis, but is lethal to C.kariyai. Less