Development of simple separation method of lactopholin from cheese whey and their functional evaluation
奶酪乳清中乳磷脂简单分离方法的建立及其功能评价
基本信息
- 批准号:10556057
- 负责人:
- 金额:$ 2.37万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B).
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Lactophorin(LP)is a major glycoprotein in the proteose-peptone fraction of whey fraction from bovine milk and constituted of two major glycopeptides(27 and 17 kDa)with other 3 to 5 minor peptides. From high content and localization in hydrophobic amino acid residues on the basis of the primary structure of 27 k peptide in LP, it is considered that LP may have a higher emulsifying functionality. This study was to develop a simple isolation method of LP from whey of cheese manufacturing and to evaluate the emulsifying activity and inhibitory activity to milk lipoprotein lipase of the obtained LP specimen. The following results were obtained in this study.1. We have established a simplified and economic isolation method of LP from cheese whey in the pilot plant scale. Cheese whey(363 kg)was concentrated to about 1/16 in its volume by passing through ultra-filter-membrane(corresponding to 500 k molecular weight)and reversed osmosis to about 1/60-70 of whey in volume, and then the concentrates containing LP were adjusted to pH4.0, heated for 30min at 95-100°C and centrifuged for 15min at 3000 rpm and the supernatant was freeze-dried. The obtained LP-enriched fraction was 54g.2. The highly purified LP was prepared by gel filtration on Sephacryl S-300 of the isolated LP-enriched fraction.3. LP-enriched fraction had the highest emulsifying activity, emulsion stability, specific surface areas and smallest median diameter of globules in emulsions prepared from 1% LP and 25% milk fat at pH7.0.4. LP-enriched fraction and purified LP had the inhibitory activity of 52% and 70%, respectively, to milk lipoprotein lipase in partly injured milk fat globule and synthetic emulsion as the substrate. Increasing purified LP increased inhibitory effect to 90%.
乳蛋白(Lactophorin,LP)是牛乳乳清蛋白中的一种主要糖蛋白,由27 kDa和17 kDa两个主要糖肽和3~5个次要多肽组成。根据Lp中27k肽的一级结构,根据Lp中疏水氨基酸残基的高含量和定位,认为Lp可能具有较高的乳化功能。本研究建立了一种从干酪生产乳清中提取乳清蛋白的简便方法,并对所得乳清蛋白样品的乳化性和乳脂酶抑制活性进行了评价。本研究取得了以下研究成果:1。建立了一种从干酪乳清中分离乳清蛋白的简便、经济的中试方法。干酪乳清(363 Kg)经超滤膜浓缩至乳清体积的1/16左右(相对分子质量为500k),反渗透至乳清体积的1/60~70左右,然后将含有乳清蛋白的浓缩液调节至pH4.0,在95~100℃下加热30min,在3000rpm下离心15min,上清液冷冻干燥。得到的富Lp组分为54g2。利用Sephacryl S-300凝胶过滤分离纯化的富Lp组分,得到高纯度的Lp。在pH值为7.0.4时,1%LP和25%乳脂制备的乳状液中,富LP组分具有最高的乳化活性、乳液稳定性、比表面积和最小的乳球中值直径。以部分受损乳脂球和合成乳状液为底物,富集组分和纯化组分对乳脂酶的抑制率分别为52%和70%。随着Lp纯度的增加,抑制率提高到90%。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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KANNO Choemon其他文献
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{{ truncateString('KANNO Choemon', 18)}}的其他基金
Development of the polymeric antibody specific to Salmonella as antibiotic substitution
开发沙门氏菌特异性聚合抗体作为抗生素替代品
- 批准号:
12460119 - 财政年份:2000
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
cDNA sequence of lactophorin and and its emulsification function
乳蛋白的cDNA序列及其乳化功能
- 批准号:
07660152 - 财政年份:1995
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Standardization of major constituents of bovine milk fat globule membrane
牛乳脂肪球膜主要成分的标准化
- 批准号:
05556047 - 财政年份:1993
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Resolution of saccharide structure and biochemical and immunological characterization of unknown glycoprotein which reacts with the antibody of milk fat globule membrane protein
糖结构的解析以及与乳脂肪球膜蛋白抗体反应的未知糖蛋白的生化和免疫学表征
- 批准号:
05660129 - 财政年份:1993
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
EMULSIFYING FUNCTION OF LACTOPHORIN AND CONDUCTION OF INHIBITION OF LIPOPROTEIN LIPASE
乳激素的乳化作用及其对脂蛋白脂肪酶的抑制作用
- 批准号:
03660291 - 财政年份:1991
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
cDNA sequence of lactophorin and and its emulsification function
乳蛋白的cDNA序列及其乳化功能
- 批准号:
07660152 - 财政年份:1995
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)