Experimental study of bone formation using vascularized periosteum and development of artificial periosteum

血管化骨膜成骨的实验研究及人工骨膜的研制

• 批准号: 10557187
• 负责人: KOBAYASHI Junji
• 金额: $ 4.16万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10557187/
• 关键词: Bone formation; Reconstructive surgery; Vascularized periosteum; rhBMP; Atelocollagen; 血管柄付き骨膜皮弁; リコンビナントBMP; 骨再建; 骨膜; 人工骨膜; BMP

Various procedures have been used to treat the difficult problems of reconstruction of bone defect following the treatment of oral and maxillofacial diseases. Recently, many pedicled flaps with bone have been used for the reconstruction, but donor sites are limited. Many authors have presented the osteogenetic capacity of vascularized, or free, periosteum, but several conclusions about bone formation by the periosteum have been showed. The purposes of this study were (1) to evaluate the osteogenesis of vascularized periosteal flap combined with atelocollagen and recombinant human bone morphogenetic protein-2 (rhBMP-2) and (2) to develop the matrix filled inside that to increase the osteogenetic capacity of periosteum.Vascularized periosteal flap of tibia of rats was elevated and atelocollagen of rhBMP-2 was rolled up by the periosteum and grafted in abdominal subcutaneous area. Histological and radiographic observations of bone formation and molecular biological examination of perioste … More um were made at 3 and 5 days and 1, 3, 5, 7 and 9 Weeks.Radiologic findings indicated that a greater amount of new bone was seen in case of rhBMP-2 implantation than that of atelocollagen. But in both cases remodeling of new bone was observed between 5 and 9 weeks after surgery. Histologic observation showed that in case of atelocollagen bone was formed with membranous ossification during 2 weeks after surgery, although in case of rhBMP-2 both endochondral and membranous ossification were seen. The rate of bone formation in case of atelocollagen was faster than that of rhBMP-2. In molecular biological examination, reproducible result could not be shown, because it was difficult to resect only periosteum for sample. The experiment of implantation of rhBMP-2/PLGA capsules (rhBMP-2 with poly (DL-lactide-co-glycolide)) to subcutaneous area of rats indicated that the role of rhBMP-2 in the extraskeletal site of rats was to induce the differentiation of mesenchymal cells into the osteoblasts. Less

口腔颌面部疾病治疗后骨缺损的修复是一个难题，目前已采用多种方法进行治疗。近年来，许多带蒂骨瓣已被用于修复，但供区有限。许多作者已经提出了血管化或游离骨膜的成骨能力，但关于骨膜成骨的几个结论已经显示出来。本研究旨在探讨带血管蒂骨膜瓣复合去端胶原和重组人骨形态发生蛋白-2（rhBMP-2）的成骨作用，以及在骨膜瓣内填充基质以增强骨膜的成骨能力。2例用骨膜卷起移植于腹部皮下。骨膜成骨的组织学、X线观察及分子生物学检测 ...更多信息 术后3、5天及1、3、5、7、9周取材，X线片显示rhBMP-2组新骨形成量明显多于去端胶原组。但在这两种情况下，在术后5至9周观察到新骨的重塑。组织学观察显示，去端胶原骨在术后2周内形成膜性成骨，而rhBMP-2则同时出现软骨内成骨和膜性成骨。去端胶原的骨形成速度快于rhBMP-2。在分子生物学检查中，由于仅切除骨膜取材困难，无法显示重复性结果。将rhBMP-2/PLGA（rhBMP-2/poly（DL-lactide-co-glycolide））胶囊植入大鼠皮下的实验表明，rhBMP-2在大鼠皮肤外的作用是诱导间充质细胞向成骨细胞分化。少

项目成果

Masatsugu Isobe, Yasuhara Yamazaki, Masahiro Mori, Kazuhiko Ishihara, Nobuo Nakabayashi, and Teruo Amagasa.: "The role of recombinant human bone morphogenetic protein-2 in PLGA capsules at an extraskeletal site of the rat"J Biomed Mater Res. 45. 36-41 (19

Masatsugu Isobe、Yasuhara Yamazaki、Masahiro Mori、Kazuhiko Ishihara、Nobuo Nakabayashi 和 Teruo Amagasa.：“重组人骨形态发生蛋白 2 在大鼠骨骼外部位 PLGA 胶囊中的作用”J Biomed Mater Res。

Masahiro Mori, et al.: "Restoration of segmental bone defects in rabbit radius by biodegradable capsules containing recombinant human bone morphogenetic protein-2"J Biomed Mater Res. 50. 191-198 (2000)

Masahiro Mori 等人：“通过含有重组人骨形态发生蛋白 2 的可生物降解胶囊修复兔桡骨节段性骨缺损”J Biomed Mater Res。

Akira Yamasaki, et al.: "BMP-induced Ectopic Bone Formation in Osteopetrotic (op/op) Mice"J. Hard Tissue Biol.. 9(1). 1-6 (2000)

Akira Yamasaki 等人：“BMP 诱导的骨质疏松 (op/op) 小鼠异位骨形成”J。

Masatsugu Isobe, et al.: "Bone Regeneration Produced in Rat Femur Defects by Polymer Capsules Containing Recombinant Human Bone Morphogenetic Protein-2"J Oral Maxillofac Surg. 57. 695-698 (1999)

Masatsugu Isobe 等：“含有重组人骨形态发生蛋白 2 的聚合物胶囊在大鼠股骨缺损中产生骨再生”J Oral Maxillofac Surg。

Masahiro Mori, Masatsugu Isobe, Yasuharu Yamazaki, Kazuhiko Ishihara, Nobuo Nakabayashi: "Restoration of segmental bone defects in rabbit radius by biodegradable capsules containing recombinant human bone morphogenetic protein-2"J. Biomed Mater Res. 50. 1

Masahiro Mori、Masatsugu Isobe、Yasuharu Yamazaki、Kazuhiko Ishihara、Nobuo Nakabayashi：“通过含有重组人骨形态发生蛋白 2 的可生物降解胶囊修复兔桡骨节段性骨缺损”J。