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Elucidation of membrane transport processes coupled with respiration mimetic reactions and development of novel membrane separation methods

阐明与呼吸模拟反应相结合的膜运输过程并开发新型膜分离方法

基本信息

批准号：
11440216
负责人：
KIHARA Sorin
金额：
$ 8.32万
依托单位：
Kyoto Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Respiration mimetic membrane reactions were realized by adopting artificial membrane systems in the absence of any enzymes and proteins, in order to get a quantitative understanding of the energetics involved in the membrane transport in a living system as well as to develop novel biomimetic separation methods of ions. The fruits obtained are as follows.New Types of Membrane Reactions Mimicking biological ProcessesConsidering a membrane system such as aqueous (W1)/membrane (M)/aqueous (W2), the reaction that a charge is incorporated from W1 into M at one site of the W1|M interface and released from M to W1 at another site of the same interface can be regarded as a membrane transport ("Parallel transport I"). Another variety of "Parallel transport" is the reaction that a charge is released from M to W1 or W2 at one site of the W1|M or W2|M interface and incorporated from W1 or W2 to M at another site of the W1|M or W2|M interface. ("Parallel transport II"). These new transports were realized by using liquid membranes.Effect of a Phospholipid Adsorbed on the Aqueous/0rganic Interface on Ion Transfers at the InterfaceThe transfer of a hydrophilic cation such as alkali metal ion, alkaline earth metal ion or H^+ from an aqueous to an organic was found to be facilitated by the phospholipid layer. When the ion was hydrophobic cation or an anion, the facilitated transfer was not observed.Evaluation of Gibbs Energy for the Transfer of a Hydrophilic Ion from an Aqueous to an Organic SolutionA method to determine the transfer energy of a hydrophilic metal ion from an aqueous solution, W, in the presence of high concentration of H^+ to an organic solution, 0,.was proposed based on the theoretical consideration of the distribution process of ions between W and 0.

为了定量地了解生物体中膜传递过程中的能量学，以及发展新型的仿生离子分离方法，在不添加任何酶和蛋白质的条件下，采用人工膜系统实现了拟呼吸膜反应.模拟生物过程的新型膜反应考虑水（W1）/膜（M）/水（W2）等膜系统，电荷从W1在W1的一个位点并入M的反应|M界面的另一个位点释放并在同一界面的另一个位点从M释放到W1，可被视为膜转运（“平行转运I”）。另一种“平行传输”是电荷在W1的一个位点从M释放到W1或W2的反应。|M或W2| M接口，并从W1或W2并入到W1另一个站点的M| M或W2| M接口。（“平行运输II”）。磷脂吸附在水/有机界面上对界面离子转移的影响研究发现磷脂层促进了亲水性阳离子如碱金属离子、碱土金属离子或H^+从水溶液向有机溶液的转移。当离子是疏水阳离子或阴离子时，没有观察到易化转移。亲水离子从水溶液到有机溶液的转移吉布斯能的评估一种确定亲水金属离子从水溶液W，在高浓度H^+存在下，到有机溶液0，基于对W和0之间的离子分布过程的理论考虑，提出了一种新的计算方法。