Elucidation of Ion Transport Process and Oscillation Processes Through a Bilayr Lipid Membrane and Its Application to Analytical Chemistry.

阐明通过双层脂质膜的离子传输过程和振荡过程及其在分析化学中的应用。

• 批准号: 07454200
• 负责人: KIHARA Sorin
• 金额: $ 4.93万
• 依托单位: KYOTO INSTITUTE OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07454200/
• 关键词: Ion transport process through bilayr lipid membrane; Ion transfer at aqueous; membrane interface; Voltammmogram for ion transfer through a membrane; Bilayr lipid membrane and liquid membrane; Spontaneous concentration of ion; Properties of ions; Pr

The correlation between the voltammogram for ion transfer through a membrane and those at two aqueous/membrane interfaces was elucidated by employing a glass cell with a liquid membrane into which an auxiliary reference electrode was inserted. The membrane transport was found to be controlled by the complementary ion transfers at two aqueous/membrane interfaces. The above methodology and concept were demonstrated to be available to analyze the characteristic reactions of the membrane, like the interdependency between two different charge transfer reactions proceeding at the respective interfaces or the oscillation of membrane potential and membrane current.The ion transport through a bilayr lipid membrane (BLM) was investigated voltammetrically. The ion transport through a BLM was found to be analogous to that through a liquid membrane when the BLM contains sufficient ions, although the BLM is very much thinner than the liquid membrane. It should be stressed that the spontaneous conden … More sation of objective ions from aqueous solution (s) into the BLM by the partition with counter ions is essential for the ion transport through BLMs.Novel oscillations of membrane current or membrane potential were observed when the transfer of Na^+ from one aqueous solution (W1) containing 0.1 M NaCl to another (W2) in the absence of Na^+ through a liquid membrane (LM) containing dilute Na^+ was forced by an applied membrane potential or membrane current, respectively. The current oscillation was induced by the addition of acetylcholine in W1, and inhibited by the addition of such hydrophobic ions as tetraalkylammonium ions or glutamate ion in W1. These oscillations were similar to those at biomembranes with the so-called "sodium channel" as regards both induction and inhibition, though the LM system did not contain any channel proteins. The mechanisms of the oscillations, the induction and the inhibitions were elucidated by referring to voltammograms for the ion transfer through a membrane as well as those at aqueous/membrane interfaces, and by taking into account the adsorption of Na^+ at the LM/W2 interface.In addition, investigations have been carried out for the electron transfer reactions at the aqueous/organic solutions interface, the coupling between the ion and electron transports through a liquid membrane and the quantitative interpretation on the ion-pair extraction equilibrium. Less

通过在玻璃池中插入辅助参比电极的液膜，阐明了离子通过膜传递的伏安图与两个水/膜界面的伏安图之间的相关性。发现膜的传输是由两个水/膜界面的互补离子转移控制的。上述方法和概念可用于分析膜的特征反应，如在各自界面上进行的两种不同电荷转移反应之间的相互依赖性或膜电位和膜电流的振荡。用伏安法研究了离子在双层脂质膜（BLM）中的传输。当BLM含有足够的离子时，离子通过BLM的传输被发现类似于通过液膜的传输，尽管BLM比液膜薄得多。需要强调的是，通过与反离子的分割，使客观离子从水溶液中更多地站在BLM中，是离子通过BLM输运的必要条件。当Na^+在没有Na^+的情况下通过含有稀释Na^+的液膜（LM）从含有0.1 M NaCl的水溶液（W1）转移到含有Na^+的水溶液（W2）时，分别受到施加的膜电位或膜电流的强迫，观察到膜电流或膜电位的新振荡。在W1中加入乙酰胆碱可诱导电流振荡，在W1中加入四烷基铵离子或谷氨酸离子等疏水离子可抑制电流振荡。这些振荡在诱导和抑制方面与生物膜上所谓的“钠通道”相似，尽管LM系统不含任何通道蛋白。通过离子通过膜和水/膜界面转移的伏安图，并考虑了Na^+在LM/W2界面上的吸附，阐明了振荡、诱导和抑制的机理。此外，还研究了水/有机溶液界面上的电子转移反应、离子和电子通过液膜传递的耦合以及离子对萃取平衡的定量解释。少

项目成果

白井理、木原壮林、吉田裕美: "液膜および脂質二分子膜を介したイオン透過反応のボルタンメトリー的解釈" 分析化学. 44. 905-920 (1995)

Osamu Shirai、Sorin Kihara、Hiromi Yoshida：“通过液膜和脂质双层膜的离子渗透反应的伏安法解释”分析化学 44. 905-920 (1995)。

H.Ohde,S.Kihara,他: "Voltammetric Study on Ion Transport across a Liquid Membrane Coupled with Electron Transport." J.Electroanal.Chem.(印刷中). (1997)

H.Ohde、S.Kihara 等人：“跨液膜离子传输与电子传输的伏安研究”（J.Electroanal.Chem）（出版中）。

S.Kihara, Y.Yoshida, et al.: "Voltammetry for Charge Transfer at the Liquid/Liquid Interface and Its Application to the Elucidation of Interfacial Reactions" Memories of the Faculty of Engineering and Design Kyoto Institute of Technology. Vol.44. 33-66 (1

S.Kihara、Y.Yoshida 等人：“液/液界面电荷转移伏安法及其在阐明界面反应中的应用”京都工业大学工程与设计学院的回忆。

H.Ohde, S.Kihara, et al.: "Voltammetric Study on Ion Transport across a Liquid Membrane Coupled with Electron Transport." J.Electroanal.Chem.(in press.).

H.Ohde、S.Kihara 等人：“跨液膜离子传输与电子传输耦合的伏安研究”。

O. Shirai, S. Kihara,他: "Ion Transfer through a Liquid Membrane or a Bilayer Lipit Membrane in the Presence of Surfficient Electrolytes." J. Electroanal. Chem.389. 61-70 (1995)

O. Shirai、S. Kihara 等人：“在存在充足电解质的情况下通过液体膜或双层 Lipit 膜的离子转移”。J. Electroanal. 61-70 (1995)。