Studies on F_1 seed production system in tomato of two pure line parents using environment-dependent restoration of male sterility

两个纯系亲本番茄F_1制种系统的环境依赖性雄性不育恢复研究

基本信息

批准号：
11460012
负责人：
MASUDA Masaharu
金额：
$ 6.53万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2002
项目状态：
已结题

项目摘要

Male sterile tomato mutant "T-4" with intact pollen or without collapsed pollen was induced by irradiation of the seeds with γ-rays. Pollen viability of "T-4" due to partial fertility restoration was investigated using male sterile mutant "T-3", with perfectly collapsed pollen, as a T-4 pollen recipient. Furthermore, mode of inheritance of male sterility and molecular marker linked to T-4 sterile gene(s) were determined.A few fruits with many seeds were observed during late September to late October, and late January to late April. Parthenocarpic fruits were observed on T-4 plants at low temperature during late October to early March. Male fertility restoration was greater at night temperature of 8℃than of 24℃, which suggested that low temperature stimulated pollen fertility restoration. In this case, self pollination was carried out by smear method. The extent of natural self-pollination was not evident, and remains a subject for the future.When heterozygous plants were selfed in six … More trials, fertile and sterile progenies segregated at a ratio 13 : 3, which suggested an involvement of fertility inhibiting gene. Fertility inhibiting gene (I) was postulated to induce male sterility in a case of homozygous recessive fertile gene "ff".On the other hand, RAPD analysis was carried out for the development of molecular markers closely linked to male sterile gene(s) of "T-4", resulting in the establishment of two markers, CMN-B30 1700 bp and CMN-B50 352 bp, both of which linked with ms gene by 8.4 cM. A RAPD band of CMN-B50 352 bp was sequenced and successfully converted to an STS marker, which can be practically utilized as a selection marker. AFLP analysis was also carried out, and a total of six polymorphic bands were detected between "First" and "T-4".From the segregation ratio mentioned above, it seems that two genotypes, a sterile type ffII and a fertile type ffii are expected. The genotype obtained from crossing them is ffIi, which is male sterility. This genotype is able to use as a mother parent for F_1 seed production. Currently, our focus is on determination of possible existence of two types of male sterility, ffII and ffIi. Less

雄性无菌番茄突变体“ T-4”带有完整的花粉或没有塌陷的花粉，是通过用γ射线照射的种子来诱导的。使用雄性无菌突变体“ T-3”研究了“ T-4”的花粉生存力，并带有完美折叠的花粉作为T-4花粉接受者。此外，确定了与T-4无菌基因相关的男性无菌和分子标记的遗传模式。在9月下旬至10月下旬以及1月下旬至4月下旬的几个水果中，很少有许多种子的水果。在10月下旬至3月初，在低温下的T-4植物上观察到parthenocarpic果实。夜间温度为8至24°时，男性生育力的恢复较大，这表明低温刺激了花粉生育力的恢复。在这种情况下，通过涂片方法进行自授粉。自然自我授粉的程度不是证据，并且仍然是未来的主题。杂合植物是自行六人……更多的试验，以13：3的比例隔离的试验，肥沃和无菌后代，这表明抑制基因的生育能力受到抑制。 Fertility inhibiting gene (I) was postulated to induce male sterility in a case of homozygous recessive fertile gene "ff".On the other hand, RAPD analysis was carried out for the development of molecular markers closely linked to male sterile gene(s) of "T-4", resulting in the establishment of two markers, CMN-B30 1700 bp and CMN-B50 352 bp, both of which linked与MS基因为8.4 cm。对CMN-B50 352 bp的RAPD带进行了测序，并成功地转换为STS标记，实际上可以用作选择标记。还进行了AFLP分析，在“第一”和“ T-4”之间总共检测到六个多态性带。从上面提到的隔离比，似乎有两种基因型，一种无菌的FFII和一种肥沃的FFIII。通过跨它们获得的基因型是FFII，这是雄性不育。该基因型能够用作F_1种子生产的母亲。当前，我们的重点是确定可能存在两种类型的男性不育，FFII和FFII。较少的