ANALYSIS OF WATER BALANCE IN CUT FLOWERS OF BULBOUS PLANTS AT GROWING AND SENESCING STAGES

球茎植物生长期和衰老期切花水分平衡分析

• 批准号: 11460016
• 负责人: IWAYA-INOUE Mari
• 金额: $ 9.41万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460016/
• 关键词: Water status; ^1H-NMR relaxation times(T1, T2); Trehalose; Turgor; Tulip (Tulipa gesneriana); Gladiolus; Post harvest; Flower senescence; ポストハーベスト; ^1H-NMRスピン・格子緩和時間(T1); グラジオラス(球根植物); 水分バランス

Water status of cut bulbous flowers was determined by ^1H-NMR spectroscopy and isopiestic psychrometers at growing and senescing stages. Treatment with trehalose was markedly effective in prolonging vase life in gladiolus and tulip flowers. Long ^1H-NMR relaxation time (T_1) of the intracellular water in petal tissues for both species showed that trehalose functioned to protect vacuolar water. Short T_2s estimated as bound water from cytoplasm and the apoplastic regions were determined by solid echo technique. In cut flowers, trehalose maintained a higher degree of water content accompanying with suppressing protein degradation and turgor loss than control petal tissues with protecting membrane integrity during growing and senescence stages. On the other hand, trehalose did not promote elongation of epidermal parenchyma cells in tulip tepal tissues, but maintained radial enlargement of the cells. Additionally, the hydraulic conductance of soybean embryos grown on trehalose-containing media became smaller than that in sucrose-containing media, suggesting that trehalose might reduce hydraulic conductance in soybean embryos, resulting in growth retardation of soybean embryos. From these results, trehalose did not act as an energy source or directly participate in osmoregulation and these characteristics distinguish it from other sugars such as sucrose.

用~1H-核磁共振波谱和等压湿度计测定了切花在生长期和衰老期的水分状况。海藻糖处理能显著延长唐菖蒲花和郁金香花的瓶插寿命。两种植物花瓣组织细胞内水的长1H-核磁共振弛豫时间(T1)表明，海藻糖具有保护液泡水的作用。用固体回波技术测定细胞质和质外体结合水的短T_2s。在切花中，海藻糖保持了较高的水分含量，同时抑制了蛋白质降解和膨压损失，在生长和衰老阶段保护了膜的完整性。另一方面，海藻糖不促进郁金香被片组织中表皮薄壁细胞的伸长，但维持细胞的径向膨大。此外，在含海藻糖的培养液中生长的大豆胚胎的水力传导性比在含蔗糖的培养液中的小，这表明海藻糖可能降低了大豆胚胎的水力传导性，从而导致大豆胚胎的生长迟缓。从这些结果来看，海藻糖不作为能量来源或直接参与渗透调节，这些特性使其有别于其他糖，如蔗糖。

项目成果

Iwaya-Inoue, M., K.Yoshimura, M.Otsubo and H.Yamasaki: "Effests of oxygen stress on ^1H-NMR relaxation time (T1) of Vigna radiata seedling"J.Fac.Agr.Kyushu Univ.. 44. 249-256 (2000)

Iwaya-Inoue，M.，K.Yoshimura，M.Otsubo 和 H.Yamasaki：“氧胁迫对荠菜幼苗 ^1H-NMR 弛豫时间 (T1) 的影响”J.Fac.Agr.Kyushu Univ.. 44

大坪繭子、井上眞理: "グラジオラスの切り花の鮮度低下における水の動態変化"園芸学会雑誌. 67(別冊)・2. 452 (1999)

Mayuko Otsubo，Mari Inoue：“由于唐菖蒲切花新鲜度下降而导致的水动态变化”，日本园艺学会杂志 67（分刊），2. 452（1999）。

Iwaya-Inoue,M.and M.Takata: "Trehalose plus chloramphenicol prolong the vase-life of tulip flowers"HortScience. 36(In press). (2001)

Iwaya-Inoue，M. 和 M.Takata：“海藻糖加氯霉素可延长郁金香花的瓶插寿命”HortScience。

Ikeda,T., M.Iwaya-Inoue, T.Fukuyama, and H.Nonami: "Trehalpse chages hydraulic conductance of tissue-cultured soybean embryos."Plant Biotechnology. (accepted). (2000)

Ikeda,T.、M.Iwaya-Inoue、T.Fukuyama 和 H.Nonami：“Trehalpse 改变组织培养大豆胚胎的水力传导。”植物生物技术。

Iwaya-Inoue,M.,K.Yoshimura,M.Otsubo and H.Yamasaki: "Effests of oxygen stress on ^1H-NMR relaxation time (T1) of Vigna radiata seedling"J.Fac.Agr.Kyushu Univ.. 44・3,4. 249-256 (2000)

Iwaya-Inoue，M.，K. Yoshimura，M. Otsubo 和 H. Yamasaki：“氧胁迫对荠菜幼苗 ^1H-NMR 弛豫时间 (T1) 的影响”J. Fac. 44。・3,4。249-256（2000）