Characterization of silica-induced protein and mechanism of microbial silica deposition

二氧化硅诱导蛋白的表征和微生物二氧化硅沉积机制

• 批准号: 11460047
• 负责人: OGATA Seiya
• 金额: $ 9.09万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460047/
• 关键词: Silica deposition; Geothermal environments; Hyperthermophilic microorganism; Thermus thermphilus; Bio-silica; Silica-induced protein; Aluminum ion; 高度好熱細菌; シリカ誘導性蛋白質; Thermus属細菌; A1イオン; 高度光熱細菌; Thermus otakeensis

A combined use of molecular ecological techniques and geochemical surveys revealed that thermophilic or hyperthermophilic microorganisms living in the geothermal environments are likely to be implicated in the formation of biogenic siliceous deposits. Electron microscopic observations indicated that numerous microorganism-like fabrics were presented in naturally occurring siliceous deposits such as siliceous sinter, geyserite, and silica scale, which suggests microbial contribution to silica precipitation. Molecular phylogenetic analysis suggested that extreme thermophilic bacteria within the genera Thermus and Hydrogenobacter are predominant components among the indigenous microbial community in siliceous deposits formed in pipes and equipment of Japanese geothermal power plants. These bacteria seem to actively contribute to the rapid formation of huge siliceous deposits. Additionally, in vitro examination suggested that Thermus cells induced the precipitation of supersaturated amorphous silica during the exponential growth phase, concomitant with the production of a specific cell envelope protein (Silica-induced protein, Sip). Sip seems to be a ferric iron-binding periplasmic protein of ABC transporter). The production of Sip was inhibited by Al ion. Dissolved silica in geothermal hot water may be a significant component in the maintenance of position and survival of microorganisms in limited niches.

分子生态学技术和地球化学调查相结合的结果表明，生活在地热环境中的嗜热或超嗜热微生物可能与生物硅质矿床的形成有关。电子显微镜观察表明，许多微生物样织物中存在于自然发生的硅质沉积物，如硅质烧结矿，硅云母，硅垢，这表明微生物的贡献二氧化硅沉淀。分子系统发育分析表明，极端嗜热菌属内的Thermus和Hydrogenolysis是日本地热发电厂的管道和设备中形成的硅质沉积物中的土著微生物群落的主要组成部分。这些细菌似乎积极地促进了巨大硅质沉积物的快速形成。此外，在体外检查表明，栖热菌细胞诱导沉淀的过饱和无定形二氧化硅在指数生长期，伴随着特定的细胞包膜蛋白（二氧化硅诱导蛋白，Sip）的生产。Sip似乎是ABC转运蛋白的三价铁结合周质蛋白）。铝离子对Sip的产生有抑制作用。地热热水中溶解的二氧化硅可能是维持有限生态位中微生物的位置和生存的重要组成部分。

项目成果

Ogata, S. and Doi, K.: "Microbial silica deposition in geothermal hot waters"Kyushu Daigaku Kenkyuu Shoukai. 20, (in Japanese). 51-52 (2003)

Ogata, S. 和 Doi, K.：“地热热水中的微生物二氧化硅沉积”九州大学研究会。

Seiya OGATA et al.: "Microbial silica deposition in geothermal hot water"Applied Microbilogy and Biotecnology. 60・2. 601-611 (2003)

Seiya OGATA 等：“地热热水中的微生物二氧化硅沉积”应用微生物学和生物技术 60・2（2003 年）。

Seiya OGATA: "Biological and Molecular Investigation of Spontaneously Developing Pocks in Streptomyces"Actinomycetologica. 15・2. 40-49 (2001)

Seiya OGATA：“链霉菌自发形成痘痕的生物学和分子研究”Actinomycetologica 15・2（2001）。

Seiya OGATA et al.: "Isolation of enterocin SE-K4-encoding plasmid and a high enterocin SE-K4 producing strain E. faecalis K-4"Journal of Bioscience and Bioengineering. 93・4. 434-436 (2002)

Seiya OGATA 等：“肠菌素 SE-K4 编码质粒和高产肠菌素 SE-K4 菌株粪肠球菌 K-4 的分离”《生物科学与生物工程杂志》93·4（2002）。

Seiya OGATA et al: "Characterization of a Phage Resistance Plasmid, pLKS, Silage-Making Lactobacillus plantarum NGRI0101"Bioscience Biotechnology and Biochemistry. 64・4. 751-756 (2000)

Seiya OGATA 等：“噬菌体抗性质粒的表征，pLKS，青贮饲料植物乳杆菌 NGRI0101”《生物科学生物技术和生物化学》64·4（2000）。