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A study on use of phytoinformation for diagnosis and plant management in greenhouses

利用植物信息进行温室诊断和植物管理的研究

基本信息

批准号：
11460120
负责人：
GOTO Eiji
金额：
$ 9.6万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460120/
关键词：
cholorophyll environmental stress fluorescence diagnosis imaging Plant

项目摘要

We have developed a new image analysis system, capable of quantitative analysis of two aspect of chlorophyll a fluorescence: i ) the changes in intensity of chlorophyll fluorescence during dark-light transitions by the induction method, and ii ) the chlorophyll fluorescence quenching by the saturation pulse method. With this system we analyzed the effects of an herbicide on the photosynthetic activity of Phaseolus vulgaris L. leaves. Furthermore, stomatal conductance and assimilation rate were measured with a diffusion porometer.Results showed that the photosynthetic injuries caused by the herbicide were detected by both fluorescence measurement methods. These injuries were also detected with the porometer as a decrease in stomatal conductance and assimilation rate. Information obtained by the induction method was effective for determining the site in the electron transport system which was damaged by the herbicide. The saturation pulse method could analyze the photosynthetic injuries more quantitatively than the induction method. However, the saturation pulse method required an evenly distributed high level of photosynthetic active radiation (PAR), which makes it difficult to apply to a large leaf area. In contrast, the induction method did not require such a high level of PAR for measurements, and was easier to use for a large leaf area. Consequently, simultaneous use of the saturation pulse method and the induction method enables detailed diagnosis of injuries of the photosynthetic system.

我们建立了一个新的图像分析系统，能够定量分析叶绿素a荧光的两个方面：一是用感应法测定暗-光转换过程中叶绿素荧光强度的变化；二是用饱和脉冲法测定叶绿素荧光的猝灭。利用该系统分析了除草剂对菜豆叶片光合作用活性的影响。此外，用扩散法测定了气孔导度和同化速率，结果表明，两种方法都能检测到除草剂引起的光合作用伤害。气孔导度和同化速率的降低也是气孔计检测到的。通过诱导法获得的信息对于确定除草剂破坏电子传递系统的位置是有效的。饱和脉冲法比诱导法能更好地定量分析光合作用伤害。然而，饱和脉冲法需要均匀分布的高水平的光合作用有效辐射(PAR)，这使得它很难应用于大的叶面积。相反，诱导法不需要如此高水平的PAR来测量，并且更容易用于较大的叶面积。因此，同时使用饱和脉冲法和诱导法能够对光合作用系统的损伤进行详细诊断。