权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Basic and prophylactic study on the toxicity of endocrine disruptors and lifestyle Testicular injury by di-(2-ethylhexyl) phthalate

邻苯二甲酸二(2-乙基己基)酯引起的内分泌干扰物毒性和生活方式对睾丸损伤的基础和预防研究

基本信息

批准号：
11470108
负责人：
JITSUNARI fumihiko
金额：
$ 4.22万
依托单位：
Kagawa Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Di-(2-ethylhexyl) phthalate (DEHP) is the most common plasticizer for plastics such as polyvinyl chloride (PVC) but suspected to be an endocrine disruptor. Recent studies have shown the toxic effects of DEHP on male reproductive organs such as testicular injury in rats in both mature and growth stages, and this injury has been suggested to be due to oxidative stress induced by mono-(2-ethylhexyl) phthalate (MEHP). Since PVC containing DEHP is widely used in manufacturing consumer goods, food containers, toys, and medical instruments, DEHP contamination is widespread, and the general population is exposed to DEHP in food, water, and air, which arouses concern about human reproductive hazards.In this study, to clarify the effects of chemical substances widely distributed in foods and beverages on the testicular toxicity of DEHP, we administered antioxidants such as vitamins E and C, caffeine, nicotinic acid, and ethanol together with DEHP to rats in the growth stage. In 4- or 5-week-old SD rats given a 1-2% DEHP containing diet for 1-2 weeks, testicular atrophy accompanied by a spermatogenesis developed. Young rats were more susceptible to the testicular toxicity of DEHP. There was a correlation between the blood or testicular MEHP concentration and the degree of DEHP-induced testicular atrophy, suggesting a dose-effect relationship between the dose of MEHP as an active toxicant and testicular injury.In 4-week-old rats given a 2% DEHP containing diet and water supplemented with vitamins E and C, testicular atrophy was definitely inhibited. Water supplemented with caffeine (0.05w/w%), nicotinic acid (0.5 w/w%), or ethanol (2.5 or 5 v/v%) also prevented testicular atrophy in 4-week-old rats given a 1% DEHP containing diet. The action mechanism of these antioxidants was unclear. However, our results suggest the involvement of antioxidant activity and free radical scavenging capacity in the inhibition of DEHP induced testicular injury.

邻苯二甲酸二（2-乙基己基）酯（DEHP）是聚氯乙烯（PVC）等塑料最常见的增塑剂，但被怀疑是一种内分泌干扰物。最近的研究表明，DEHP对雄性生殖器官有毒性作用，例如在成熟和生长阶段的大鼠睾丸损伤，这种损伤被认为是由于邻苯二甲酸单（2-乙基己基）酯（MEHP）诱导的氧化应激所致。由于含DEHP的PVC广泛用于制造消费品、食品容器、玩具和医疗器械，DEHP污染十分普遍，一般人群会接触到食物、水和空气中的DEHP，这引起了人们对人类生殖危害的关注，在本研究中，为了阐明食品和饮料中广泛分布的化学物质对DEHP睾丸毒性的影响，我们给处于生长阶段的大鼠服用抗氧化剂，如维生素E和C、咖啡因、烟酸和乙醇以及DEHP。在4或5周龄SD大鼠中，给予含1-2% DEHP的饲料1-2周，睾丸萎缩伴精子发生。年轻大鼠更容易受到DEHP的睾丸毒性。血液或睾丸MEHP浓度与DEHP诱导的睾丸萎缩程度之间存在相关性，表明MEHP作为活性毒物的剂量与睾丸损伤之间存在剂量-效应关系。在4周龄大鼠中，给予含2% DEHP的饮食和补充维生素E和C的水，睾丸萎缩明显受到抑制。补充有咖啡因（0.05w/w%）、烟酸（0.5w/w%）或乙醇（2.5或5v/v%）的水也可预防给予含1%DEHP饮食的4周龄大鼠的睾丸萎缩。这些抗氧化剂的作用机制尚不清楚。然而，我们的研究结果表明，抗氧化活性和自由基清除能力参与抑制DEHP诱导的睾丸损伤。