MECHANISMS OF PESTICIDE-INDUCED TESTICULAR ATROPHY

农药引起睾丸萎缩的机制

• 批准号: 2153992
• 负责人: Rex Allen Hess
• 金额: $ 7.96万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2153992
• 关键词: Sertoli cells; adenosinetriphosphatase; antifungal agents; atrophy; benzimidazoles; densitometry; dosage; electron microscopy; enzyme mechanism; epididymis; epithelium; fertility; histology; image processing; insecticide biological effect; laboratory rat; morphology; occupational hazard; pesticide interaction; spermatogenesis; statistics /biometry; testis disorder; tissue /cell culture; video recording system

This research will determine the mechanisms by which the fungicides benomyl and carbendazim induce testicular atrophy. Benomyl is a systemic benzimidazole fungicide that is used widely on numerous food crops and ornamentals. Carbendazim, also a benzimidazole fungicide, is a metabolite and natural breakdown product of benomyl. Both of these chemicals are known male reproductive toxicants. Benzimidazole exposures induce focal and total testicular atrophy, which cause infertility in male rats. However, the mechanisms leading to infertility remain unknown. Preliminary studies indicate that occlusion of the efferent ductules of the testis may be a major factor in the development of testicular atrophy. The proposed experiments will determine: a. acute dose-dependent changes in the testis and efferent ductules after exposure to benomyl and carbendazim; b. whether carbendazim's acute effects on the testis and efferent ductules are independent; c. the effect of carbendazim on Sertoli cell morphology in the testis; d. whether carbendazim disrupts the structure and function of ciliated and nonciliated cells of the ductal epithelium; e. if the testicular injury is reversible, depending upon the extent of efferent ductule or seminiferous tubule damage; and f. the relationship of testicular and ductal damage multiple exposures to low dosages of carbendazim. The results of this research will provide valuable information regarding the mechanisms of male infertility produced by the benzimidazole class of compounds, which will allow for more informed decisions of occupational risk to males from the wide spread use of these fungicides.

这项研究将确定杀菌剂的作用机制

项目成果

Rooster testicular germ cells and epididymal sperm contain P450 aromatase.

公鸡睾丸生殖细胞和附睾精子中含有P450芳香酶。

• DOI: 10.1095/biolreprod53.6.1259
• 发表时间: 1995
• 期刊: Biology of reproduction
• 影响因子: 3.6
• 作者: Kwon,S;Hess,RA;Bunick,D;Nitta,H;Janulis,L;Osawa,Y;Bahr,JM
• 通讯作者: Bahr,JM

Computer tracking of germ cells in the cycle of the seminiferous epithelium and prediction of changes in cycle duration in animals commonly used in reproductive biology and toxicology.

• DOI: 10.1002/j.1939-4640.1992.tb00297.x
• 发表时间: 1992-05
• 期刊: Journal of andrology
• 作者: Rex A. Hess;Paul Chen

Adult testicular enlargement induced by neonatal hypothyroidism is accompanied by increased Sertoli and germ cell numbers.

新生儿甲状腺功能减退引起的成人睾丸肿大伴随着支持细胞和生殖细胞数量的增加。

• DOI: 10.1210/endo.132.6.8504761
• 发表时间: 1993
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: Hess,RA;Cooke,PS;Bunick,D;Kirby,JD
• 通讯作者: Kirby,JD

Acute and long-term effects of a single dose of the fungicide carbendazim (methyl 2-benzimidazole carbamate) on the male reproductive system in the rat.

• DOI: 10.1002/j.1939-4640.1992.tb00345.x
• 发表时间: 1992-11
• 期刊: Journal of andrology
• 作者: Masaaki Nakai;Rex A. Hess;Billy J. Moore;Robert F. Guttroff;L. Strader;Ralph E. Linder

Germ cells of the mouse testis express P450 aromatase.

• DOI: 10.1210/endo.132.3.8440194
• 发表时间: 1993-03
• 期刊: Endocrinology
• 影响因子: 4.8
• 作者: H. Nitta;D. Bunick;R. Hess;L. Janulis;S. Newton;C. Millette;Y. Osawa;Y. Shizuta;K. Toda;J. Bahr