Study on transcutaneous immunization using peptides of melanomd entigens with high affinity to MHC

MHC高亲和力黑色素抗原肽经皮免疫研究

• 批准号: 11470181
• 负责人: SAIDA Toshiaki
• 金额: $ 9.28万
• 依托单位: Shinshu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470181/
• 关键词: Malignant melnomd; Immunotherapy; Antigen peptide; Transcutaneous immunization; 抗原ペプチド; 経皮感作; 樹状細胞; 細胞障害性T細胞

In our previous studey, we investigated induction of cytotoxic T lymphocytes ( CTL ) against melanoma cells by means of percutaneous application of epitope peptide of melanoma antigens. Application of mouse-gp100 peptide ( EGSRNQDWL ) but not human gp100 peptide ( KVPPNQDWL ) on tape-stripped skin of ear and abdomen of C57BL/6 mice induced slight immune response against B16 mouse melanoma, which was confirmed by CTL assay.This year, we investigated whether this treatment could induce delayed type hypersensitivity reaction ( DTH ) and inhibit growth of B16 melanoma in vivo. Application of the mouse-gp100 peptide on the tapestripped skin of the ear and abdomen of the mice induced DTH reaction, which was detected by foodpad test. However, growth of B16 melanoma was not significantly inhibited in the mice treated with the application of mouse-gp100 peptide.Seo et al reported strong inhibitory effect on the growth of B16 melanoma with almost the same treatment in this murine melanoma system. However, we could not confirm the excellent results in our study. Further study may be necessary to determine the effect of this unique method of immunization.

在我们以前的研究中，我们研究了通过经皮应用黑色素瘤抗原的表位肽来诱导针对黑色素瘤细胞的细胞毒性T淋巴细胞（CTL）。小鼠gp 100肽（EGSRNQDWL）而非人gp 100肽（KVPPNQDWL）应用于C57 BL/6小鼠耳和腹部胶带剥离皮肤上诱导了针对B16小鼠黑色素瘤的轻微免疫应答，CTL试验证实了这一点。将小鼠-gp 100肽应用于小鼠耳和腹部的胶带剥离的皮肤上，诱导DTH反应，通过食物垫试验检测DTH反应。然而，在用小鼠-gp 100肽处理的小鼠中，B16黑素瘤的生长没有被显著抑制。Seo等人报道了在该鼠黑素瘤系统中，用几乎相同的处理对B16黑素瘤的生长具有强烈的抑制作用。然而，我们无法证实我们研究中的出色结果。可能需要进一步研究以确定这种独特的免疫方法的效果。

项目成果

Ichikawa,T.,et al: "Increased synthesis of hyaluronate enhances motility of human melanoma cells"J.Invest.Dermatol. 113(6). 935-939 (1999)

Ichikawa, T., 等人：“增加透明质酸的合成可增强人类黑色素瘤细胞的运动性”J.Invest.Dermatol。

Kubo,H., et al: "Sequential chemoimmunotherapy with cisplatin, IFN-β, and IL-2 inhibits the growth of B16-F1 melanoma in syngeneic mice"Melanoma Res. 10. 223-229 (2000)

Kubo, H., 等人：“使用顺铂、IFN-β 和 IL-2 的序贯化学免疫疗法抑制同系小鼠中 B16-F1 黑色素瘤的生长”Melanoma Res. 10. 223-229 (2000)

斎田俊明: "皮膚癌の多段階発癌"Molecular Medicine. 36(4). 416-422 (1999)

Toshiaki Saida：“皮肤癌的多步骤致癌”《分子医学》36(4) (1999)。

Kageshita,T., Saida,T. et al: "Growth inhibition of human malignant melanoma transfected with the human IFN-β gene by means of catiuonic liposomes"Melanoma Res. 11. 337-342 (2001)

Kageshita，T.，Saida，T.等：“通过阳离子脂质体转染人IFN-β基因的人恶性黑色素瘤的生长抑制”Melanoma Res. 11. 337-342 (2001)

Kageshita, T., et al.: "Growth inhibition of human malignant melanoma transefcted with human IFN-β gene by means of cationic liposomes"Melanoma Res.. 11. 337-342 (2001)

Kageshita，T.，等：“通过阳离子脂质体转染人 IFN-β 基因的人恶性黑色素瘤的生长抑制”Melanoma Res.. 11. 337-342 (2001)