EFFECTS OF VOLATILE ANESTHETICS ON PH ACTIVITY AND ISCHEMIC-REPERFUSION INJURY IN MYOCARDIAL CELLS

挥发性麻醉药对心肌细胞PH活性和缺血再灌注损伤的影响

• 批准号: 11470320
• 负责人: KUMAZAWA Teruo
• 金额: $ 9.09万
• 依托单位: UNIVERSITY OF YAMANASHI (FACULTY OF MEDICINE)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470320/
• 关键词: MYOCYTES; PH ACTIVITY; VOLATILE ANESTHETICS; REPERFUSION INJURY; H^+交換系; Na+; H+交換系

We studied the effects of volatile anesthetics on pH activity in myocardial cells during ischemic-reperfusion injury. In this study, we examined pH activity, Ca2+ transients, and contractility in isolated rabbit ventricular myocytes. Intracellular H+ concentration (pHi), Na+ concentration ([Na+]i) and Ca2+ concentration ([Ca2+]i) were measured with the fluorescent pH indicators (BCFCE), Na+ indicators (SBFI) and Ca2+ indicators (Fluo-3), respectively. Contractility was assessed from changes in cell shortening during field stimulation. Experiments were performed at extracellular pH 7.4 in the presence of HEPES buffer. Our findings demonstrate that 1) halothane significantly decreased contractility compared with isoflurane, 2) halothane and isoflurane attenuate the magnitude in decreases in pHi during reperfusion, 3) halothane and isoflurane attenuate the magnitude in decreases in pHi during ischemic period. It is suggested that a reduction of excessive intracellular hydrogen after reperfusion caused by halothane and isoflurane may be beneficial on myocardial function following reperfusion in the intact rabbit myocardium.

我们研究了吸入麻醉药对缺血-再灌注损伤心肌细胞pH活性的影响。在这项研究中，我们研究了pH值的活动，Ca 2+瞬变，并在离体兔心室肌细胞的收缩。分别用荧光pH指示剂（BCFCE）、Na+指示剂（SBFI）和Ca 2+指示剂（Fluo-3）测定细胞内H+浓度（pHi）、Na+浓度（[Na+]i）和Ca 2+浓度（[Ca 2 +]i）。收缩性根据场刺激期间细胞缩短的变化进行评估。在HEPES缓冲液存在下，在细胞外pH 7.4下进行实验。我们的研究结果表明：1）与异氟烷相比，氟烷显著降低收缩力，2）氟烷和异氟烷减弱再灌注期间pHi降低的幅度，3）氟烷和异氟烷减弱缺血期间pHi降低的幅度。提示氟烷和异氟烷引起的再灌注后细胞内过量氢的减少可能对完整兔心肌再灌注后的心肌功能有益。