Development of preventive method for dental caries by using phosphoryl-oligosaccharides

开发利用磷酰低聚糖预防龋齿的方法

• 批准号: 11470384
• 负责人: IMAI Susumu
• 金额: $ 4.67万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470384/
• 关键词: phosphoryl-oligosaccharides; mutans streptococci; dental caaries; acid fermentation; artificial mouth system; demineralization; エナメル質脱灰

The purpose of this study was to develop the preventive methods for dental caries by using functional new oligosaccharides which are not only less fermentable by mutans streptococci, but also inhibitory against dental caries through the suppression of organic acids production and promotion of re-mineralization. Phosphoryl-oligosaccharides (POs) were produced from potato starch by using alfa-amylase. Effect of POs on the precipitation of calcium-phosphate was examined. POs inhibited the precipitation of calcium-phosphate and promoted the solubility of calcium. POs itself were not fermented by mutans streptococci such as S.mutans and S.sobrinus. Moreover, POs inhibited pH decrease of medium due to the fermentation of sucrose by mutans streptococci in dose-dependent manner. It was suggested that inhibition of pH decrease by POs was ascribed to buffering action of POs. In artificial mouth system, POs inhibited the accumulation of artificial biofilm on the enamel slab surface, pH decrease underneath the artificial biofilm and de-mineralization of enamel by mutans streptococci. It was confirmed from these results that POs possessed appropriate characters for the caries preventive agents, and was applicable to various measures for the prevention of dental caries.

本研究的目的是开发一种新的功能性低聚糖，这种低聚糖不仅不易被变形链球菌发酵，而且通过抑制有机酸的产生和促进再矿化来抑制龋齿。以马铃薯淀粉为原料，利用α -淀粉酶制备了低聚磷酸。考察了POs对磷酸钙沉淀的影响。POs抑制了磷酸钙的沉淀，促进了钙的溶解度。POs本身不被变形链球菌如s.a mutans和s.a sobrinus发酵。此外，POs还能抑制变形链球菌发酵蔗糖引起的培养基pH降低，且呈剂量依赖性。在人工口腔系统中，POs抑制了牙釉质板表面人工生物膜的积累、人工生物膜下pH的降低以及变形链球菌对牙釉质的脱矿作用。结果表明，POs具有合适的防龋剂特性，适用于各种防龋措施。

项目成果

Feasibility of eradication of mutans streptococci from oral cavities.

从口腔中根除变形链球菌的可行性。

• 发表时间: 2004
• 期刊: Journal of oral science 46
• 作者: Nomura Y;Takeuchi H;Kaneko N;Matin;K;Iguchi R;Toyoshima Y;Kono Y;Ikemi T;Imai S;Nisizawa T;Fukushima K;Hanada N.
• 通讯作者: Hanada N.

釜阪寛, 今井 奨 他: "馬鈴薯デンプン由来リン酸化オリゴ糖のミュータンスレンサ球菌への影響"口腔衛生学会雑誌. 52(1). 66-71 (2002)

Hiroshi Kamasaka、Sho Imai 等人：“源自马铃薯淀粉的磷酸化寡糖对变形链球菌的影响”《口腔健康协会杂志》52(1) (2002)。

Inhibitory effect of a self-derived peptide on glucosyltransferase of Streptococcus mutans -: Possible novel anticaries measures

• DOI: 10.1074/jbc.274.22.15797
• 发表时间: 1999-05-28
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Eto, A;Saido, TC;Hanada, N
• 通讯作者: Hanada, N

Clinical study of mutans streptococci using 3DS and monoclonal antibodies.

使用 3DS 和单克隆抗体对变形链球菌进行临床研究。

• 发表时间: 2001
• 期刊: Jpn J.Infect.Dis. 54
• 作者: Takeuchi H;Fukushima K;Senpuku H;Nomura Y;Kaneko N;Yano A;Morita E;Imai S;Nisizawa T;Kono Y;Ikemi T;Toyoshima Y;Hanada N.
• 通讯作者: Hanada N.

New dental drug delivery system for removing mutans streptococci from the oral cavity : effect on oral microbial flora.

用于从口腔中去除变形链球菌的新型牙科药物输送系统：对口腔微生物菌群的影响。

• 发表时间: 2000
• 期刊: Jpn J.Infect.Dis. 53
• 作者: Takeuchi H;Senpuku H;Matin K;Kaneko N;Yusa N;Yoshikawa E;Ida H;Imai S;Nisizawa T;Abei Y;Kono Y;Ikemi T;Toyoshima Y;Fukushima K;Hanada N.
• 通讯作者: Hanada N.