Development of preventive method for dental caries by using phosphoryl-oligosaccharides

本研究的目的是开发一种新的功能性低聚糖，这种低聚糖不仅不易被变形链球菌发酵，而且通过抑制有机酸的产生和促进再矿化来抑制龋齿。以马铃薯淀粉为原料，利用α -淀粉酶制备了低聚磷酸。考察了POs对磷酸钙沉淀的影响。POs抑制了磷酸钙的沉淀，促进了钙的溶解度。POs本身不被变形链球菌如s.a mutans和s.a sobrinus发酵。此外，POs还能抑制变形链球菌发酵蔗糖引起的培养基pH降低，且呈剂量依赖性。在人工口腔系统中，POs抑制了牙釉质板表面人工生物膜的积累、人工生物膜下pH的降低以及变形链球菌对牙釉质的脱矿作用。结果表明，POs具有合适的防龋剂特性，适用于各种防龋措施。

The purpose of this study was to develop the preventive methods for dental caries by using functional new oligosaccharides which are not only less fermentable by mutans streptococci, but also inhibitory against dental caries through the suppression of organic acids production and promotion of re-mineralization. Phosphoryl-oligosaccharides (POs) were produced from potato starch by using alfa-amylase. Effect of POs on the precipitation of calcium-phosphate was examined. POs inhibited the precipitation of calcium-phosphate and promoted the solubility of calcium. POs itself were not fermented by mutans streptococci such as S.mutans and S.sobrinus. Moreover, POs inhibited pH decrease of medium due to the fermentation of sucrose by mutans streptococci in dose-dependent manner. It was suggested that inhibition of pH decrease by POs was ascribed to buffering action of POs. In artificial mouth system, POs inhibited the accumulation of artificial biofilm on the enamel slab surface, pH decrease underneath the artificial biofilm and de-mineralization of enamel by mutans streptococci. It was confirmed from these results that POs possessed appropriate characters for the caries preventive agents, and was applicable to various measures for the prevention of dental caries.