Construction of mutanase chimer ic enzyme by domain shuffling and degradation of biofilm by the chimeric enzyme

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Cariogenic mutans streptococci produce water-insoluble glucan involving in biofilm formation from sucrose as a substrate. The purpose of this study was to construct chimeric enzyme composed of dextranase and mutanase that degrade α-1, 6- and α-1, 3-glucosidic bond, respectively. Dextranase gene and mutanase gene were cloned in plasmid and the chimeric gene was successfully constructed in the expression vector and the resulting enzyme hydrolyzed effectively dextran and mutan simultaneously. It is desired to improve the chimeric enzyme to highly functional one in the future.