Study of the occurrence mechanism of adhesion tissues in the temporomandibular joint

颞下颌关节粘连组织发生机制的研究

• 批准号: 11470433
• 负责人: MIZUTANI Hideki
• 金额: $ 3.84万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470433/
• 关键词: temporomandibular joint; adhesion; culture; electron microscopy; fluorescence staining; actin

To characterize the adhesions in internal derangement (ID) of the temporomandibular joint (TMJ), we used a gel contraction model to investigate the ability of fibroblast-like cells derived from human normal articular disc (D), synovium (S) and two types of adhesion tissue of ID in the TMJ (band-like adhesion [BA], pseudowall-like adhesion [PW]).Sequential measurements of gel diameter for 12 days showed that six kinds of cells contracted CG continuously. Among TMJ-related cells, PW had the greatest contraction potency. Differences in ability of the contraction potency may reflect their proliferative potential, migratory activity, production of and response to cytokines, and synthesis of extracellular matrix products. The order of contraction potency is ; NS>PW>BA>D/GF>SArrangement of cells mtd ECM components were examined in two types of adhesions by TEM, SEM and rhodamine-labeled phalloidine staining. In BA, there were a few fibroblast-like cells with thick filopodia processes and orde … More rly arranged collagen bundles were prominent. The collagen bundles ran parallel to direction in which the adhesion was extended. In PW, there were a lot of active fibroblast-like cells with many fine filopodia processes and randomly arranged collagen bundles were mainly seen. A part of pseudowall-like adhesion, calcified collagen fibrils were observed in the extracellular matrix. The adhesions that showed the highest resistance during arthroscopic biopsy consisted of calcifiecl collagen fibrils. This structure in internal derangement may strongly inhibit movement of the disc and joint.In addition the effects of tranilast, N-(3,4-dimethoxycinnamoyl) anthranilic acid, a widely used antiallergy drug in Japan on the gel contraction process was also investigated. The degree of inhibition induced by tranilast was not equal in the two types of fibroblast-like cell ; BA showing a much greater response to tranilast than PW.The reasons for the difference in magnitude of response to tranilast are unclear, although it may reflect differences in the level of the receptor expression for tranilast or response to cytokines by each cell type. Less

为了表征颞下颌关节（TMJ）内部紊乱（ID）中的粘连，我们使用凝胶收缩模型来研究来自人类正常关节盘（D）、滑膜（S）和TMJ中ID的两种粘连组织（带状粘连[BA]、假壁样粘连[PW]）的成纤维细胞样细胞的能力。顺序测量 12天凝胶直径的变化表明，6种细胞CG持续收缩。在 TMJ 相关细胞中，PW 具有最大的收缩能力。收缩能力的差异可能反映了它们的增殖潜力、迁移活性、细胞因子的产生和反应以及细胞外基质产物的合成。收缩效力的顺序是； NS>PW>BA>D/GF>S 通过 TEM、SEM 和罗丹明标记的鬼笔环肽染色检查两种类型粘连中细胞 mtd ECM 成分的排列。 BA中可见少量纤维母细胞样细胞，丝状伪足粗大，有序排列的胶原束突出。胶原束平行于粘附延伸的方向。 PW中可见大量活跃的成纤维细胞样细胞，有许多细小的丝状伪足，主要可见随机排列的胶原束。细胞外基质中可见部分假壁样粘连、钙化的胶原原纤维。在关节镜活检中显示出最高阻力的粘连由钙化胶原纤维组成。这种内部紊乱的结构可能会强烈抑制椎间盘和关节的运动。此外，还研究了日本广泛使用的抗过敏药物曲尼司特、N-(3,4-二甲氧基肉桂酰)邻氨基苯甲酸对凝胶收缩过程的影响。曲尼司特对两类成纤维细胞样细胞的抑制程度并不相同； BA对曲尼司特的反应比PW大得多。对曲尼司特的反应程度不同的原因尚不清楚，尽管它可能反映了曲尼司特受体表达水平的差异或每种细胞类型对细胞因子的反应。较少的

项目成果

Katsuhiro Senga: "Ultrastructural study on ashesions in internal derangement of the temporomandibular joint"Journal of Oral and Maxillofacial Surgery. 57. 165-170 (1999)

Katsuhiro Senga：“颞下颌关节内部紊乱的超微结构研究”口腔颌面外科杂志。

Senga K., Mizutani H., Kobayashi M., Ueda M.: "Ultrastructural study on adhesions in internal derangement of the temporomandibular joint"J Oral Maxillofac Surg. 57. 165-170 (1999)

Senga K.、Mizutani H.、Kobayashi M.、Ueda M.：“颞下颌关节内部紊乱中粘连的超微结构研究”J Oral Maxillofac Surg。

Katsuhiro Senga: "Ultrastructural study on adhesions in internal derangement of the temporomandibular joint"Journal of Oral and Maxillofacial Surgery. 57. 165-170 (1999)

Katsuhiro Senga：“颞下颌关节内部紊乱中粘连的超微结构研究”口腔颌面外科杂志。