Study of the occurrence mechanism of adhesion tissues in the temporomandibular joint

为了表征颞下颌关节（TMJ）内部紊乱（ID）的粘连，我们采用凝胶收缩模型研究了来源于人正常关节盘(D)、滑膜(S)和两种类型的关节紊乱的粘附组织（带状粘连[BA]、假性粘连[PW]）的成纤维细胞样细胞在TMJ中的粘附能力。连续测量凝胶直径12天，6种细胞连续收缩CG。在tmj相关细胞中，PW的收缩力最大。收缩能力的差异可能反映了它们的增殖潜能、迁移活性、细胞因子的产生和应答以及细胞外基质产物的合成。收缩力的顺序为；采用透射电镜（TEM）、扫描电镜（SEM）和罗丹明标记法酞定染色检测两种粘附类型下细胞mtd和ECM组分的排列情况。BA内可见少量成纤维细胞样细胞，丝状突较粗，排列有序，胶原束排列较整齐。胶原束平行于粘连延伸的方向。PW内可见大量活跃的成纤维细胞样细胞，细丝足突多，胶原束多呈随机排列。细胞外基质中可见部分假性粘连，胶原原纤维钙化。关节镜活检时显示阻力最大的粘连是钙化胶原原纤维。这种内部紊乱的结构可能会强烈地抑制椎间盘和关节的运动。此外，还研究了日本广泛使用的抗过敏药物曲尼司特N-（3,4-二甲氧基肉桂酰）邻氨基苯酸对凝胶收缩过程的影响。曲尼司特对两类成纤维细胞样细胞的抑制程度不相等；BA对曲尼司特的反应比PW大得多。对曲尼司特的反应程度差异的原因尚不清楚，尽管它可能反映了每种细胞类型对曲尼司特的受体表达水平或对细胞因子的反应的差异。少

To characterize the adhesions in internal derangement (ID) of the temporomandibular joint (TMJ), we used a gel contraction model to investigate the ability of fibroblast-like cells derived from human normal articular disc (D), synovium (S) and two types of adhesion tissue of ID in the TMJ (band-like adhesion [BA], pseudowall-like adhesion [PW]).Sequential measurements of gel diameter for 12 days showed that six kinds of cells contracted CG continuously. Among TMJ-related cells, PW had the greatest contraction potency. Differences in ability of the contraction potency may reflect their proliferative potential, migratory activity, production of and response to cytokines, and synthesis of extracellular matrix products. The order of contraction potency is ; NS>PW>BA>D/GF>SArrangement of cells mtd ECM components were examined in two types of adhesions by TEM, SEM and rhodamine-labeled phalloidine staining. In BA, there were a few fibroblast-like cells with thick filopodia processes and orde … More rly arranged collagen bundles were prominent. The collagen bundles ran parallel to direction in which the adhesion was extended. In PW, there were a lot of active fibroblast-like cells with many fine filopodia processes and randomly arranged collagen bundles were mainly seen. A part of pseudowall-like adhesion, calcified collagen fibrils were observed in the extracellular matrix. The adhesions that showed the highest resistance during arthroscopic biopsy consisted of calcifiecl collagen fibrils. This structure in internal derangement may strongly inhibit movement of the disc and joint.In addition the effects of tranilast, N-(3,4-dimethoxycinnamoyl) anthranilic acid, a widely used antiallergy drug in Japan on the gel contraction process was also investigated. The degree of inhibition induced by tranilast was not equal in the two types of fibroblast-like cell ; BA showing a much greater response to tranilast than PW.The reasons for the difference in magnitude of response to tranilast are unclear, although it may reflect differences in the level of the receptor expression for tranilast or response to cytokines by each cell type. Less