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Central distribution of neuronal cell bodies innervating the levator veli palatini muscle and its myosin heavy chain isoform expression pattern - for development of a new palate repair procedure -

支配提腭肌的神经元细胞体的中央分布及其肌球蛋白重链亚型表达模式 - 用于开发新的上颚修复程序 -

基本信息

批准号：
11470444
负责人：
YASUDA Kouichi
金额：
$ 9.02万
依托单位：
MATSUMOTO DENTAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

The levator veli palatini (LVP) muscle has a very important role in the complex functions regulating velopharyngeal mechanisms. Previous studies indicate that the LVP muscle is innervated by the glossopharyngeal nerve which is contained efferent and afferent component, and LVP motoneurons are localized in the ambiguous nucleus, and afferen neurons are projected to nucleus solitary tract. In this study, we shall present ; First, in more tooled on the central distribution of the LVP motoneurons and afferent terminals, and investigated location of LVP afferent neuronal cell bodies by using electrophysiological and horseradish peroxidase (HRP)- tracing techniques ; Second, serial muscle cross-sections using monoclonal antibodies specific for myosin heavy chain to characterize the muscle fibers of the LVP muscle HRP-labeled motoneurons in the ambiguous nucleus were separated two rostrocaudally components which were distinguish between rostral division and caudal division, in the rostral division correspond the Botzinger Complex and caudal division correspond rostral ventral respiration group. HRP-labeled afferent neuronal cell bodies were observed in the glossopharyngo-vagal complex ganglion, and HRP-labeled afferent terminals were observed in the bilateral lateral region of nucleus solitary tract. THe LVP muscle was shown to be composed of two muscle compartments by myosin heavy chain isoform expression pattern ; one showed no staining for slow myosin heavy chain isoform but reacted positively for the fast myosin heavy chain isoform, while the other was stained positively for both fast and slow myosin heavy chain isoform. These results suggest it was minutely invested relationship between the myosin heavy chain isoform expression pattern and central distribution of LVP motoneurons or central projections of afferent neurons with regard to active both inspiration and expiration of the LVP muscle.

腭帆提肌（LVP）在调节咽运动机制的复杂功能中起着非常重要的作用。以往的研究表明，LVP肌受舌咽神经支配，舌咽神经含有传出和传入成分，LVP运动神经元位于疑核，传入神经元投射到孤束核。本研究首先对LVP运动神经元和传入终末的中枢分布作了进一步的研究，并应用电生理和辣根过氧化物酶（HRP）示踪技术对LVP传入神经元胞体的定位进行了研究;第二、使用肌球蛋白重链特异性单克隆抗体的连续肌肉横截面，以表征LVP肌肉HRP的肌纤维。疑核内标记的运动神经元在吻尾侧分为两个区，分别为吻侧区和尾侧区，吻侧区对应Botzinger复合体，尾侧区对应吻侧腹侧呼吸群。在舌咽-迷走神经复合节内可见HRP标记的传入神经元胞体，在孤束核两侧外侧区可见HRP标记的传入终末。肌球蛋白重链亚型表达模式显示LVP肌肉由两个肌室组成;一个肌室对慢肌球蛋白重链亚型无染色，但对快肌球蛋白重链亚型呈阳性反应，而另一个肌室对快肌球蛋白重链亚型和慢肌球蛋白重链亚型均呈阳性反应。这些结果表明，肌球蛋白重链亚型的表达模式与LVP运动神经元的中枢分布或传入神经元的中枢投射之间存在着密切的关系。