Postnatal change of mastication- related and respiration-related central nervous systems

咀嚼相关和呼吸相关中枢神经系统的出生后变化

• 批准号: 15390627
• 负责人: YASUDA Kouichi
• 金额: $ 9.41万
• 依托单位: Matsumoto Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390627/
• 关键词: mastication; respiration; ageing; jaw muscles; swallowing; シナプス; 前運動ニューロン

Trigeminal motoneurons are involved in a variety of oral motor activities, including mastication and breathing, which must be adapted to postnatal environmental change. Serotonin has both an excitatory and an inhibitory effect on trigeminal motor function, whereas substance P has mainly an excitatory effect. In the present study, we measured the density of serotonin- and substance P-immunoreactive nerve terminals in the trigeminal motor nucleus and the area 300 μm surrounding it in rats from embryonic day 19 to postnatal day 70. The density of these terminals gradually increased from embryonic day 19 to postnatal day 7 and decreased thereafter. The density was greatest in the ventromedial subnucleus of the trigeminal motor nucleus at embryonic day 19 and postnatal day 0 and in the area 300 μm surrounding trigeminal motor nucleus at postnatal day 4 and older. Two-color fluorescence immunohistochemistry was used to identify nerve processes immunoreactive for both substance P and serotonin. Approximately 90% of serotonergic terminals also contained substance P at all ages examined, which suggests that the physiological function of terminals in which these neurotransmitters are colocalized is similar throughout development.Postnatal change of expression of NK1,5HT1A and 5HT1B receptors was not recognized in the trigeminal motor nucleus and the area 300μm surrounding trigeminal motor nucleus

三叉神经运动神经元参与多种口腔运动活动，包括咀嚼和呼吸，必须适应产后环境的变化。 5-羟色胺对三叉神经运动功能既有兴奋作用，又有抑制作用，而P物质主要具有兴奋作用。在本研究中，我们测量了大鼠从胚胎第19天到出生后第70天三叉神经运动核及其周围300μm区域中血清素和P物质免疫反应性神经末梢的密度。这些末梢的密度从胚胎第19天到出生后第7天逐渐增加，此后逐渐减少。胚胎第19天和出生后第0天三叉神经运动核的腹内侧亚核密度最大，出生后第4天及以上时三叉神经运动核周围300μm的区域密度最大。双色荧光免疫组织化学用于识别对 P 物质和血清素均具有免疫反应的神经过程。在所检查的所有年龄段中，大约 90% 的血清素末梢也含有 P 物质，这表明这些神经递质共定位的末梢的生理功能在整个发育过程中是相似的。在三叉神经运动核和三叉神经运动核周围 300μm 区域中未发现 NK1、5HT1A 和 5HT1B 受体表达的出生后变化。

项目成果

Colocalization of serotonin and substance P in the postnatal rat trigeminal motor nucleus and its surroundings

出生后大鼠三叉神经运动核及其周围血清素和 P 物质的共定位

• 发表时间: 2006
• 期刊: International journal of Developmental neuroscience 24
• 作者: Tanaka;S.;Minami;M.;Murakami;Y.;Ogiwara;T.;et al.;Sakagami H;Hirata A;Matsuura T. et al.;Masaaki Nakamura et al.
• 通讯作者: Masaaki Nakamura et al.

Evidence of parasympathetic postganglionic neurons in the rat hypoglossal nerve trunk

大鼠舌下神经干中副交感节后神经元的证据

• 发表时间: 2003
• 期刊: Experimental Brain Research 153・3
• 作者: Yasuda K;et al.
• 通讯作者: et al.

K.Yasuda, et al.: "Central distribution of neuronal cell bodies innervating the levator veli palatini muscle and associated pattern of myosin heavy chain isoform expression in rat"Brain Research. 968. 80-88 (2003)

K.Yasuda 等人：“大鼠提腭帆肌神经元细胞体的中心分布和肌球蛋白重链亚型表达的相关模式”大脑研究。

K.Yasuda, et al.: "Evidence of parasympathetic postganglionic neurons in the rat hypoglossal nerve trunk"Experimental Brain Research. 153. 302-309 (2003)

K.Yasuda 等人：“大鼠舌下神经干中副交感神经节后神经元的证据”实验脑研究。