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The effect of treadnill running training on neural plasticity in the hippocampus in juverile rats.

跑步训练对幼年大鼠海马神经可塑性的影响。

基本信息

批准号：
11480004
负责人：
SOYA Hideaki
金额：
$ 9.02万
依托单位：
Institute of Health and Sports Sciences, University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

In this study, we investigated following three experiments. First, we examined whether acute running at the lactate threshold (LT) acts on the dentate gyms (DG) to induce BDNF mRNA expression, we used a running model using Wistar male rats (Experiment 1). Second, we examined whether chronic running training either or above the LT specifically causes up-regulation of BDNF mRNA in the DG (Experiment 2). Finally, we estimated how the same types of exercise affects neurogeniesis in DG. In the experiment 1, exercise induced BDNF mRNA expressions, but these expressions were not observed depending on exercise intensity. These results suggest that the hippocampus would be activated even by low exercise intensity in acute single bout of exercise.In the experiment 2, rats were subjected to Naive (N: sedentary), and to 4 weeks of running training either below-(4W: walking) or supra-(4T, trotting) LT The effects of these training on hippocampal BDNF mRNA and morris water maze were examined. Unlike … More the experiment 1, the BDNF mRNA levels in the DG were observed to be up-regulated in rats in 41 but not in 4W Since plasma corticosterone levels and adrenal weight in 4T were significantly higher than those in N, chronic exericse increased hippocampal neuroplastisity though stress response occurred concurrently. There were no significant differences in spatial learning in the morris water maze. Thus we failed to confirm the hippocampal neuroplastisity by behavioral approach.These results suggest that acute LT running has the potential role of inducing BDNF mRNA expression in the DG. Adaptive up-regulation of BDNF mRNA expression can be induced by light running below the LT in acute running, and also can be induced by high intensity running (supra-LT) in chronic running. In addition, chronic running tended to be induced neurogenesis (Experiment 3), indicating exercise training would be increased hippocampal plastisity.It is worth examining that exercise induced hippocampal plastisity can be detected by morris water maze again, or some other behavioral approaches. Less

在本研究中，我们进行了以下三个实验。首先，我们使用Wistar雄性大鼠的跑步模型（实验1），研究了乳酸阈（LT）下的急性跑步是否对齿状回（DG）产生作用以诱导BDNF mRNA表达。第二，我们研究了长期跑步训练是否会导致DG中BDNF mRNA的上调（实验2）。最后，我们估计了相同类型的运动如何影响DG的神经发生。在实验1中，运动诱导了BDNF mRNA的表达，但这些表达并不依赖于运动强度。实验2：大鼠进行Naive（N：久坐）训练和4周的低于LT（4 W：步行）或高于LT（4 T，小跑）的跑步训练，观察这些训练对海马BDNF mRNA和Morris水迷宫的影响。不像 ...更多信息实验1：41周时海马DG区BDNF mRNA表达上调，但4 W时无明显变化。由于4 T时血浆皮质酮水平和肾上腺重量显著高于N时，慢性运动虽然同时存在应激反应，但仍能增加海马神经可塑性。在Morris水迷宫中的空间学习能力无显著差异。这些结果提示，急性LT跑可诱导DG内BDNF mRNA的表达。在急性跑中，低于LT的轻度跑可诱导大鼠脑源性神经营养因子mRNA表达的适应性上调，在慢性跑中，高强度跑（supra-LT）也可诱导大鼠脑源性神经营养因子mRNA表达的适应性上调。另外，长期跑步有诱导神经发生的趋势（实验3），表明运动训练可以增加海马的可塑性，值得验证的是，运动诱导的海马可塑性是否可以通过Morris水迷宫或其他行为学方法再次检测。少