The Fundamental Research on the Prophylaxis of Cyclospora Infectious Disease in Water Supply

供水环孢子虫传染病防治的基础研究

• 批准号: 11480154
• 负责人: ISHIBASHI Yoshinobu
• 金额: $ 4.8万
• 依托单位: Tohoku Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11480154/
• 关键词: Cyclospora; Traveler's diarrhea; Protozoa; Chlorine tolerant; Water supply; 磁気ビーズ回収法

Cyclospora is the same newly-tisen protozoa as Cryptosporidium. Its infection route is a contaminated tap water and foods and cyclospriasis shows symptoms of water-like diarrhea. Unlike Cryptosporidium a mass outbreak has not occurred in Japan. However, seven cases of cyclospriasis are reported as import infectious disease. There is a possibility that Cyclospora takes a risk of mass outbreak through water supply. Generally, research of Cyclospora is just started and knowledge is not enough. The purpose of this research was carried out to get the fundamental knowledge for cyclospriasis prevention.As the beginning of the study, information retrieval, detection methods of cyclospora oocyst and infection and multiplication using cancer cells were investigated. It took three years for the last experiment. In the final year, cyclospora was identified as C. cayetanensis using genetic analysis. Existence of Cyclospora and Cryptospridium oocyst and Giarrdia cyst in aquatic environment and wastewater, ultraviolet irradiation for inactivation etc. were examined.On the other hand, we went to Nepal several times, where disease rate is high, and made an inspection of the state of infection.

环孢子虫是与隐孢子虫相同的新发现的原生动物。它的感染途径是被污染的自来水和食物，而环孢子虫病表现出水样腹泻的症状。与隐孢子虫不同，日本没有发生大规模爆发。然而，7例环孢子虫病报告为输入性传染病。环孢子虫有可能通过供水大规模爆发。目前对环孢子虫的研究还处于起步阶段，认识还不够。本研究的目的是为环孢子虫病的防治提供基础知识，首先对环孢子虫卵囊的信息检索、检测方法以及利用癌细胞感染和增殖进行了研究。上一次实验花了三年时间。在最后一年，环孢霉被鉴定为C。cayetanensis使用遗传分析。考察了水环境和废水中环孢子虫、隐孢子虫卵囊和贾第鞭毛虫包囊的存在情况，紫外线照射灭活等。另一方面，我们多次前往发病率高的尼泊尔，对感染状况进行了考察。

项目成果

木村憲司, 石橋良信他: "新興感染症の原因となる病原性原虫サイクロスポーラの検出方法"用水と廃水印刷中. 44(4). (2002)

Kenji Kimura、Yoshinobu Ishibashi 等人：“检测引起新兴传染病的致病性原生动物的方法”水和废水出版社 44(4)。

国包章一: "水道におけるクリプトスポリジウム対策"厚生. 54(10). 17-19 (1999)

Shoichi Kuniba：“供水中隐孢子虫的对策”Kosei 54(10)。

Kimura K.: "Sterilization of Cryptosporidium"Zosui gijyutu. Vol.26, No.3. 18-26 (2000)

Kimura K.：“隐孢子虫的灭菌”Zosui gijyutu。

木村 憲司, 石橋 良信他: "サイクロスポーラの検出法"第52回全国水道研究発表会. 632-633 (2001)

Kenji Kimura，Yoshinobu Ishibashi，等：“环孢子虫的检测方法”第52届全国供水研究会议632-633（2001）。

国包章一,北澤弘美,眞柄泰基: "水道水源におけるクリプトスポリジウム及びジアルジア実態調査結果の解析"水道協会雑誌. 68(4). 22-31 (1999)

Shoichi Kuniwa、Hiromi Kitazawa、Yasuki Makara：“自来水源中隐孢子虫和贾第鞭毛虫的调查结果分析”日本自来水协会杂志 68(4) (1999)。