Regulation of cell division by ubiquitin-dependent proteolysis and by spindle assembly checkpoint

分析了后期促进复合物（APC）的调控机制。结果发现，APC活性不仅通过其调节因子Cdc 20和Cdh 1与APC的结合来调节，而且还通过APC本身和Cdc 20和Cdh 1的磷酸化来调节。APC 10/Doc 1被鉴定为APC亚基之一。Slk是plk的上游激酶，调控有丝分裂的各个阶段。鉴定了一种新的动粒蛋白CENP-H。CENP-H在整个细胞周期中仅定位于内动粒板的活性动粒处，并形成多聚体，与MCAK结合，MCAK被认为是调节染色体分离的分子。CENP-H被发现是基本的动粒结构所必需的，因为CENP-H的去除导致CENP-C的去除，CENP-C是动粒中的关键基本成分之一。

Regulatory mechanism of anaphase promoting complex (APC) was analyzed. It was found that APC activity was regulated not only by binding of its regulatory factors Cdc20 and Cdh1 to APC, but also by phosphorylation of APC itself and Cdc20 and Cdh1. APC10/Doc1 was identified as one of the APC subunits. Slk was identified as an upstream kinase of plk, which regulates various stages of mitosis. A novel kinetochore protein CENP-H was identified. The CENP-H was specifically localized in inner kinetochore plate only at active kinetochore throughout the cell cycle, and formed multimer by itself and bound MCAK which was thought to regulate choromosome separation. CENP-H was found to be essential for basic kinetochore structure, since delection of CENP-H resulted in elimination of CENP-C, one of the key basic constituents in kinetochore.