A Comprehensive Study on Intrusion and Cooperation of Genomes

基因组入侵与合作的综合研究

基本信息

批准号：
11490007
负责人：
SHIMA Akihiro
金额：
$ 8.45万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

Endonuclease domain of telomere associated non-LTR retrotransposon, TRAS1 of the silkworm could recognize and cleave the telomeric repeat (TTAGG) n. Yeast PI-SceI was used as a model system to study molecular mechanism of gene homing. Gene organization, replication system and expression of extrachromosomal DNAs from plant mycoplasma mutant lines and its relationship with host specificity and pathogenicity were analyzed, DEVDase activation was correlated with cyclobutane pyrimidine dimers, but the UVC-induced apoptosis occurred independent of the caspase-3-like activation in the Medaka cells. The molecular mechanism of emergence of polysemous codon was clarified by analyzing leucine acceptance of mutant tRNAs synthesized in vitro. Crossing of the Medaka of the HNI strain to those of the T5 tester strain revealed that the female germ cells of the HNI strain showed a remarkably higher spontaneous total mutation rates compared to that found for those of the males, however, the spontaneous … More viable mutation rates were lower in females than males, indicating that the spontaneous mutations in female germ cells are associated with larger deletions of the genome. Several STS makers were isolated from the dioecious plant, Silene latifolia, and then single-pollen analysis and isolation of a BAC clone were accomplished. Mechanism of inhibition and acclimation of photosynthetic machinery to environmental stress and its significance were studied from the evolutional point of view. Dynamics and roles of cortical microtubules in higher plant cells were investigated using living cells stably expressing GFP-tubulin proteins. The regulatory genes, Hox, Otx, and the engrailed were cloned from a sea lily, Metacrinus rotundus, for analyzing the expression pattern. A nine-residue peptide inhibiting ecdysteroid biosynthesis in the prothoracic glands was isolated from the larval brains of the silkworm, Bombyx mori. Ligand specificity of olfactory receptors was investigated in a functionally-reconstituted system. Differentiation of the intestinal crypt cells to the epithelium was characterized using IEC-6 cells transfected with a homeobox gene, Cdx-2. Proliferation and differentiation of neural stem cells are regulated by a tumor suppressor, p53. Novel members of visual pigment genes and their genomic organizations were revealed in New World primates and zebrafish. Less

端粒相关的非LTR逆转座子的核酸内切酶结构域，蚕的TRAS1可以识别并清除远程射击重复（TTAGG）n。酵母Pi-SCEI被用作研究基因归巢的分子机制的模型系统。分析了植物支原体突变系中的基因组织，复制系统和表达其与宿主特异性和致病性的关系，Devdase的激活与环丁烷嘧啶二聚体相关，但UVC诱导的凋亡与Caspase-3型细胞无关。通过分析在体外合成的突变体TRNA的接受度来阐明聚合物密码子出现的分子机制。 HNI菌株与T5测试剂菌株的MEDAKA的穿越表明，与男性相比，HNI菌株的女性生殖细胞表现出的赞助商总突变率明显更高，但对于男性的雌性菌株，赞助子自发性的……在女性中比男性较高的女性相比，具有更大的女性细胞，而较高的是，具有较大的女性细胞是基因生成细胞的。从二元植物的锡烯latifolia中分离出几个STS制造商，然后完成了单支化合物分析和BAC克隆的分离。从进化的角度研究了抑制和适应光合机制及其意义的机制。使用顽固地表达GFP微管蛋白的活细胞研究了皮质微管在高植物细胞中的动力学和作用。调节基因，HOX，OTX和插入的基因是从海百合（Metacrinus Rotundus）克隆的，用于分析表达模式。从蚕虫的幼虫大脑中分离出了抑制原始腺体中胚胎生物合成的九种残基肽。在功能固定的系统中研究了嗅觉受体的配体特异性。使用带有同源基因基因CDX-2翻译的IEC-6细胞来表征肠道加密细胞对上皮细胞的分化。神经干细胞的增殖和分化受肿瘤抑制剂p53调节。视觉色素基因及其基因组组织的新颖成员在新世界灵长类动物和斑马鱼中得到了揭示。较少的